鉴定与cuproptosis相关的分子亚型作为生物标志物,以区分儿童的活跃结核病和潜伏结核病
Liang Chen1, Jie Hua2, Xiaopu He3
1Department of Infectious Diseases, Nanjing Lishui People's Hospital, Zhongda Hospital Lishui Branch, Southeast University, No.86, Chongwen Street, Lishui District, Nanjing City, 211002, China. chenliang1995@sina.com.
BMC genomics
|July 1, 2023
概括
性结核,一种新的细胞死亡途径,与儿童活跃结核病 (ATB) 有关. 研究人员确定了与cuproptosis相关的亚型,并开发了一个预测模型来区分ATB和潜感染 (LTBI).
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物标志物发现发现
背景情况:
- 细胞死亡机制在活动性结核病 (ATB) 从潜伏性感染 (LTBI) 的进展中至关重要.
- 新发现的编程细胞死亡,即cuproptosis,与各种疾病病理有关.
- 确定特定的分子亚型对于区分儿科ATB和LTBI至关重要.
研究的目的:
- 确定与cuproptosis相关的分子亚型作为潜在的生物标志物,以区分儿科ATB和LTBI.
- 分析cuproptosis调节剂,免疫特征和儿科结核病的疾病状况之间的关联.
- 开发和验证儿童ATB与LTBI的预测模型.
主要方法:
- 在儿科ATB和LTBI队列中分析cuproptosis调节者的基因表达特征和免疫特征 (GSE39939).
- 达成共识的聚类定义了基于差异表达的质亡相关基因 (DE-CRGs) 的分子亚型.
- 针对特定亚型的DEG和机器学习模型 (SVM,XGB,RF,GLM) 的权重基因共同表达网络分析 (WGCNA),用于使用测试数据集 (GSE39940) 进行预测准确性验证.
主要成果:
- 确定了9个DE-CRG,将活性免疫反应与ATB联系起来.
- 在儿科ATB患者中定义了两个不同的与cuproptosis相关的分子亚型.
- 一个5基因支持向量机 (SVM) 模型在测试数据集中在区分ATB和LTBI方面表现出高准确度 (AUC=0.905).
结论:
- 杯症可能在儿科Mycobacterium结核病感染的免疫病理学中发挥作用.
- 基于cuproptosis亚型的强大的预测模型可以可靠地区分儿科ATB和LTBI.
- 这项研究为改善儿童结核病诊断提供了潜在的新生物标志物.
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