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Hsa_circ_0102899 在非小细胞肺癌中促进表皮层-介质细胞转换

YanFei Wang¹, JieMing He², YongXue Liang³

¹Department of Thoracic Surgery, Yan'an Affiliated Hospital of Kunming Medical University, No. 245, East Renmin Road, Panlong District, Kunming City, 650051, Yunnan Province, China.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

|July 1, 2023

概括

循环RNA (circRNA) hsa_circ_0102899促进非小细胞肺癌 (NSCLC) 的进展,通过增强上皮细胞-介质细胞转变 (EMT) 和转移. 这种circRNA调节miR-885-5p/EIF4G2轴,驱动NSCLC细胞中的恶性行为.

科学领域:

分子瘤学分子瘤学
癌症生物学癌症生物学
在RNA生物学,RNA生物学.

背景情况:

循环RNAs (circRNAs) 越来越多地被认为是它们在癌症发展中的作用.
非小细胞肺癌 (NSCLC) 是全球癌症相关死亡的主要原因.
在NSCLC中,circRNAs的特定功能,如hsa_circ_0102899,仍然需要完全阐明.

研究的目的:

研究hsa_circ_0102899在非小细胞肺癌 (NSCLC) 中的功能作用.
探索circ_0102899影响NSCLC进展的潜在分子机制.

主要方法:

评估NSCLC组织中的circ_0102899表达,并将其与临床特征相关联.
利用A549细胞检查circ_0102899调制对增殖,细胞亡,迁移和上皮层-介质细胞过渡 (EMT) 的影响.
使用瘤异种移植模型验证了体内效应,并研究了涉及微RNA和基因的调节机制.

主要成果:

发现circ_0102899在NSCLC组织中高度表达,并与瘤特征相关.
Knockdown of circ_0102899 在体内显著抑制了NSCLC细胞生长,EMT和瘤形成.
确定了一个调节轴,其中circ_0102899与miR-885-5p结合,准真核细胞翻译启动因子4γ2 (EIF4G2),从而促进恶性行为.

结论:

在NSCLC中,circ_0102899作为一种关键的致癌性circRNA.
该circ_0102899/miR-885-5p/EIF4G2通路促进EMT和NSCLC中的转移.
circ_0102899代表了NSCLC治疗的潜在治疗标.

关键词:

皮质介质酶过渡皮质介质过渡细胞转化启动因子 4γ2 非小细胞肺癌是非小细胞肺癌.环_010289999 在线阅读微型RNA-885-5p是什么意思

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