相关实验视频

Updated: Jul 24, 2025

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

Published on: October 17, 2020

使用空间转录组学剖析精子干细胞利基

Shreya Rajachandran¹, Xin Zhang¹, Qiqi Cao¹

¹Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA; Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX, USA.

|July 2, 2023

概括

精子干细胞 (SSCs) 依赖于丸的穴来生产精子. 这项研究绘制了利基互动的地图,揭示了小鼠中的类蛋白和人类中的以弗林-A1,并将炎症与糖尿病引起的丸损伤联系起来.

科学领域:

生殖生物学生殖生物学
干细胞生物学干细胞生物学
分子和细胞生物学分子和细胞生物学

背景情况:

精子干细胞 (SSC) 对于整个生命中持续的精子生产至关重要.
SSCs居住在专门的丸中,调节它们的自我更新和分化.
在SSC的内部,复杂的分子和细胞交叉声并未完全被理解.

研究的目的:

为了全面剖析SSC的分子,细胞和空间特征.
为了空间地绘制小鼠和人类丸中的联体受体 (LR) 相互作用.
调查基相互作用在丸健康和疾病 (包括糖尿病引起的损伤) 中的作用.

主要方法:

整合空间转录学,先进的计算分析和功能测试.
系统地解剖SSC微观环境的利基.
在哺乳动物丸中的联结体受体相互作用的空间分辨率映射.

主要成果:

通过综合酶受体识别了类蛋白信号传递,这对小鼠的SSC功能至关重要.
发现了以弗林-A1作为人类SSC功能的潜在调节剂.
揭示了与炎症相关的LR相互作用的改变空间分布与糖尿病诱导的丸损伤有关.

结论:

这项研究为SSC在健康和疾病领域的利基组织提供了系统层面的理解.
这些发现突出了对SSC调节至关重要的特定LR相互作用 (聚氨基/合成,氨酸-A1).
这项研究阐明了炎症对丸功能的影响,并为未来关于男性不孕症和再生医学的研究提供了框架.

关键词:

科普:干细胞研究

更多相关视频

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

Published on: July 12, 2017

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

相关概念视频

Stem Cell Niche

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...