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中脑星细胞衍生的神经营养因子促进轴突再生和在坐骨神经受伤后的运动功能的恢复

Hyemi Lee¹, Seungyeon Jeong¹, Hyun-Ju Kim²

¹Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea.

Biochemical and biophysical research communications

|July 2, 2023

概括

通过介质细胞干细胞 (MSC) 输送的介质脑星细胞衍生神经营养因子 (MANF) 显著增强了神经再生和外周神经损伤后的功能恢复,超过了胎盘生长因子 (PlGF).

科学领域:

再生医学是一种再生医学.
神经科学是一个神经科学.
生物技术是生物技术.

背景情况:

周围神经受伤往往会导致由于轴突再生有限而导致持续的感觉和运动缺陷.
当前的治疗策略往往无法恢复全功能,这凸显了需要新的再生方法的需要.

研究的目的:

为了评估中干细胞 (MSCs) 的疗效,这些细胞被设计成表达中干细胞中干细胞衍生神经变因子 (MANF) 或胎盘生长因子 (PlGF),用于外围神经修复.
在坐骨神经损伤模型中,通过人类脱细胞化神经 (HDN) 传递的MANF和PlGF的再生潜力进行比较.

主要方法:

编码MANF或PlGF的重组腺相关病毒 (AAV) 载体被用于转导MSCs (hMSC-MANF,hMSC-PlGF).
工程MSC被移植HDN进入老鼠的坐骨神经损伤模型.
通过行为测试来评估功能恢复,并使用免疫组织化学和G比分析量化神经再生.

主要成果:

与对照组相比,hMSC-MANF和hMSC-PlGF都促进了轴突再生和增加了轴突数量.
与plgf相比,manf显著加速了感官和运动功能恢复.
与hMSC-PlGF相比,hMSC-MANF治疗导致轴突更厚,骨髓化改善.

结论:

使用AAV-MANF工程的MSC的移植代表了增强外围神经损伤中的功能恢复和轴突再生的有希望的策略.
在促进神经修复和功能恢复方面,MANF表现出比PLGF更高的疗效.

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