IP3R1调节猪卵细胞成熟和早期胚胎发育中的平衡
Chang Zhang1, Lepeng Gao1, Deyi Wu1
1College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street 2888, Changchun, 130118, China.
Theriogenology
|July 2, 2023
概括
伊诺西1,4,5-三酸盐受体 (IP3Rs) 对于在猪卵细胞成熟期间维持平衡至关重要. 失去IP3R1功能会损害卵细胞的成熟,导致氧化应激增加和亡.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生理学 细胞生理学
- 生物化学 生物化学
背景情况:
- 平衡对于卵细胞成熟和早期胚胎发育至关重要.
- 伊诺西1,4,5-三酸盐受体 (IP3Rs) 调节内网膜和线粒体之间的动态.
- 在正常的猪卵细胞中IP3Rs的作用在很大程度上仍未被探索.
研究的目的:
- 研究IP3R1在猪卵细胞成熟和早期胚胎发育中的表达和功能.
- 阐明IP3R1在调节卵细胞半转化过程中的平衡中的作用.
主要方法:
- 在猪卵细胞中离合过程中IP3R1表达的分析.
- 评估卵细胞成熟,积细胞扩张和极体挤出.
- 研究内网膜和线粒体之间的流.
- 测量反应性氧物种 (ROS) 和亡水平.
主要成果:
- IP3R1的表达稳定,并在猪卵细胞化过程中局限于皮质,在MII阶段形成皮质集群.
- IP3R1活动的丧失导致卵细胞成熟失败,累积细胞扩张受损,极体挤出受阻.
- IP3R1通过IP3R1-GRP75-VDAC1通道调节平衡,将ER和线粒体连接起来.
- 抑制IP3R1导致ER功能障碍,线粒体过载,氧化应激,ROS增加和亡.
结论:
- 在猪卵细胞成熟期间,IP3R1对于维持平衡至关重要.
- IP3R1在调节ER-线粒体转移,防止氧化应激和亡方面发挥着至关重要的作用.
- 针对IP3R1功能对于改善卵细胞质量和早期胚胎发育至关重要.
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