相关实验视频
Updated: Jul 24, 2025

11:14
Modeling Paracrine Noncanonical Wnt Signaling In Vitro
Published on: December 10, 2021
1.6K
通过调节WNT5A,FOXE1有助于牛皮的发展
Meng Liu1, Guanfei Zhang2, Ziyang Wang1
1Department of Dermatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
The Journal of investigative dermatology
|July 2, 2023
概括
一种蛋白质FOXE1在牛皮中受到上调,导致角质细胞的增殖和炎症. 减少FOXE1可以改善牛皮的症状,这表明它.
科学领域:
- 皮肤病学 皮肤病学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 牛皮是一种慢性炎症性皮肤疾病,具有复杂的,尚未完全理解的发病机制.
- 关键特征包括角质细胞的过度增殖和免疫细胞的透.
研究的目的:
- 为了研究分叉箱家族蛋白E1 (FOXE1) 在牛皮的病变发生过程中的作用.
- 探索FOXE1作为牛皮的潜在治疗点.
主要方法:
- 分析患者皮肤中的FOXE1表达和一种因米基莫德诱导的小鼠模型.
- 在体外研究中,使用带有FOXE1倒置和过度表达的角质细胞 (KCs).
- 用RNA测序来识别下游的效应因子.
- 在体内研究使用小毛RNAs的lentiviral输送小毛RNAs向FOXE1在小鼠.
主要成果:
- 在病变性牛皮皮肤和KCs中,FOXE1的表达增加.
- 通过促进G1/S过渡和激活ERK1/2信号,FOXE1促进了KC的扩散.
- 减少FOXE1 knockdown可以减少促炎性细胞因子 (IL-1β,IL-6,TNF-α) 的产生.
- 确定WNT5A是FOXE1的下游效应因子,调解KC增殖和炎症.
- 在小鼠中,FOXE1的减弱会改善牛皮样皮肤炎.
结论:
- FOXE1通过促进KC增殖和炎症,在牛皮病原发生过程中发挥着重要作用.
- 福克斯1及其下游效应器WNT5A是治疗牛皮的潜在治疗点.
相关概念视频
Canonical Wnt Signaling Pathway
8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
Non-Canonical Wnt Signaling Pathways
7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K
Pleiotropy
40.7K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
40.7K
T Cell Types and Functions
1.1K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.1K
TGF - β Signaling Pathway
7.5K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.5K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.3K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.3K
