用添加剂修改以点/粉为基础的珠子,以改善用于农业应用的细菌细菌封装
Marina Momesso Lopes1, Christiane Abreu de Oliveira-Paiva2, Cristiane Sanchez Farinas3
1National Nanotechnology Laboratory for Agribusiness (LNNA), Embrapa Instrumentation, 13560-970 São Carlos, SP, Brazil; Graduate Program of Biotechnology, Federal University of São Carlos, 13565-905 São Carlos, SP, Brazil.
International journal of biological macromolecules
|July 2, 2023
概括
将 Bacillus subtilis 封装在带有添加剂的点丁/粉珠中,可以提高其在压力下的生存能力. 这些生物肥料配方显示出高可行性和稳定性,满足微生物接种剂的要求.
科学领域:
- 农业科学 农业科学
- 微生物学 微生物学
- 材料科学 材料科学 材料科学
背景情况:
- 基于细菌的生物肥料提供可持续的农业生产力.
- 有效的配方对于保护微生物细胞免受环境压力因素的保护至关重要.
- 使用点/粉基质的离子热是一种可行的封装策略.
研究的目的:
- 为了研究添加剂 (MMT,ATP,PEG,CMC) 对Bacillus subtilis封装的果/粉珠属性的影响.
- 评估这些配方对环境压力的保护作用.
- 评估封装生物肥料的长期可行性和储存稳定性.
主要方法:
- 使用含有各种添加剂的点/粉珠进行离子化凝.
- 里叶变换红外光谱 (FTIR) 和X射线衍射 (XRD) 用于材料的特征.
- 扫描电子显微镜 (SEM) 和X射线微断层扫描用于形态分析.
- 封装后,暴露于杀菌剂和紫外线辐射以及储存期间进行活力测定.
主要成果:
- FTIR证实了pectin-Ca2+相互作用;XRD表明了良好的粘土分散.
- SEM和微观断层学揭示了由添加剂影响的独特的珠子形态.
- 所有配方都实现了高初始活力 (>10^10 CFU g-1),释放概况各异.
- 素/粉,素/粉-MMT,素/粉-CMC珠子提供了优越的真菌杀菌剂保护.
- 丁/粉-ATP珠显示出最佳的紫外线保护.
- 所有配方在储存六个月后都保持 >10^9 CFU g-1.
结论:
- 添加剂显著影响了点/粉珠的物理特性和Bacillus subtilis的保护能力.
- 优化的配方可以提高生物肥料对杀菌剂和紫外线辐射等环境挑战的适应性.
- 开发的封装方法确保了长期稳定性,符合有效微生物接种剂的标准.
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