一项研究评估核在良性甲状腺病变与RET/PTC1和RET/PTC3基因转位的关联
Basavaraj Rangalakshmi Ashwini1, Chandran Nirmala2, Muthuvelu Natarajan2
1Department of Pathology, Bangalore Medical College and Research Institute, Bengaluru, Karnataka, India. dr_ashwinibr@yahoo.co.in.
Thyroid research
|July 2, 2023
概括
核纹在36.8%的良性甲状腺病变 (BTL) 中出现,其存在可能表明RET/PTC基因转位. 病理学家应考虑密切关注BTL与核沟的患者,特别是哈希莫托甲状腺炎 (HT).
科学领域:
- 内分泌学 在内分泌学.
- 在瘤学瘤学.
- 病理学 病理学 病理学
背景情况:
- 乳头甲状腺癌 (PTC) 是最常见的甲状腺恶性瘤,经常表现出核沟.
- 在良性甲状腺病变 (BTL) 中也发现了核沟,这给诊断带来了挑战.
- RET/PTC基因转位与PTC相关,并且可能存在于BTL.
研究的目的:
- 确定BTL (结节,哈希莫托甲状腺炎,卵泡腺瘤) 中核槽的频率.
- 评估BTL和RET/PTC1和RET/PTC3基因转位中的核沟之间的关联.
主要方法:
- 来自87个BTL的H&E染色的FFPE组织块的分析.
- 使用0-3分数系统对核槽的评估.
- 具有核槽的细胞的激光捕获微剖析,然后进行RNA提取,cDNA转换和RET/PTC1和RET/PTC3转位的RQ-PCR.
主要成果:
- 在36.8%的BTL中 (37个案例中32个) 观察到核槽.
- 在核槽数量和RET/PTC基因转位之间发现了显著的关联 (p=0.001).
- 哈西莫托甲状腺炎与RET/PTC基因转位显著相关 (p=0.038).
结论:
- 在BTL中核槽,特别是当与增加的核大小和改变的形状相关时,表明潜在的底层RET/PTC基因转位.
- 这些发现支持病理学家建议对BTL呈现核沟的患者进行密切跟踪,特别是在哈西莫托甲状腺炎的情况下.
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