来自大豆β-甘氨酸的酸诱导人类外围多态核白细胞的迁移
Kazuki Nagashima1, Norihiko Fujii2, Saori Oka3
1Laboratory of Clinical Pharmaceutics, Faculty of Pharma-Sciences, Teikyo University.
Biological & pharmaceutical bulletin
|July 2, 2023
概括
大豆蛋白,MITL和MITLAIPVNKPGR,刺激人类免疫细胞的迁移. 这些来自食物的可以通过甲基受体1 (FPR1) 途径激活多态核白细胞.
科学领域:
- 免疫学 免疫学 免疫学
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
背景情况:
- 食物衍生的具有多样化的生物活性.
- 食物对人类免疫细胞运动性的影响仍然在很大程度上未被探索.
- 了解这些相互作用对于营养免疫学至关重要.
研究的目的:
- 为了研究大豆蛋白β-凝聚素衍生的酸对人类多态核白细胞 (PMN) 运动性的影响.
- 阐明诱导免疫细胞迁移的潜在分子机制.
主要方法:
- 消化大豆β-凝聚素使用体内酶 (trypsin,胰腺弹性酶) 产生.
- 评估分化的人类HL-60细胞和初级PMN的诱导迁移.
- 分析甲基受体 (FPR) 的mRNA表达 1.
- 研究细胞内反应和受体脱敏.
主要成果:
- 大豆酸MITL和MITLAIPVNKPGR显著诱导PMN和HL-60细胞迁移,以剂量和时间依赖的方式.
- 迁移在二甲基cAMP (Bt2cAMP) 分化HL-60细胞中更为明显,FPR1mRNA表达增加.
- 诱导的迁移被FPR抑制剂 (Boc-MLP) 和百日咳毒素 (PTX) 抑制,涉及到FPR1.
- MITLAIPVNKPGR触发了细胞内释放,该释放被fMLP无敏化,证实了FPR1的参与.
结论:
- 大豆衍生MITL和MITLAIPVNKPGR作为人类PMN的化学吸引剂.
- 迁移通过甲基受体1 (FPR1) 途径进行介导.
- 这些发现凸显了食物衍生的在调节免疫细胞功能的潜力.
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