在人体血中进行脂去除以增强化学暴露物质
Kalliroi Sdougkou1, Hongyu Xie1, Stefano Papazian1,2
1Department of Environmental Science, Science for Life Laboratory, Stockholm University, Stockholm 106 91, Sweden.
Environmental science & technology
|July 3, 2023
概括
这项研究为人体血提供了一个优化的化学曝光学协议,通过去除脂物质,显著改善了对环境污染物的检测. 增强的方法允许对77种优先分析物的敏感分析,并发现以前无法检测到的物质.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 人体血中的化学暴露面临挑战,因为内源化合物和环境污染物之间的度差异是内源化合物的1000倍.
- 脂,丰富的内源性分子,干扰血中低度环境化学物质的敏感检测.
研究的目的:
- 通过改进的脂去除步骤,验证针对人体血的优化化学暴露学协议.
- 提高针对性和非针对性液体染色学-高分辨率质谱学用于环境污染物分析的灵敏度和范围.
主要方法:
- 开发并验证了一种化学曝光学协议,其中包括一个优化的脂去除步骤.
- 利用向和非向液体染色学高分辨率质谱仪进行敏感的多类分析.
- 分析了200μL血用于目标分析和100μL血用于个人成人血分析 (n=34).
主要成果:
- 在增加注射体积和最小矩阵效应的77个优先分析物中,达到0.05 ng/mL的中位数量化方法极限 (MLOQ).
- 在去除脂后,非脂的平均总信号强度提高了6倍 (正模式) 和4倍 (负模式).
- 检测到109% (积极模式) 和28% (负模式) 更多的非脂分子特征,使得以前无法检测到的物质的注释成为可能,包括广泛的uron暴露和PFAS前体.
结论:
- 优化的脂去除显著提高了人体血中化学暴露物的检测能力.
- 该协议允许对优先环境分析的敏感量化和发现新型暴露.
- 这种方法是一个有价值的,可扩展的工具,用于大规模的人类暴露组研究的代谢学.
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