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在EcPV2阳性皮状细胞癌的雌马上皮质-介质细胞过渡
Livia De Paolis1, Federico Armando2, Vittoria Montemurro3
1Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D'Aosta, National Reference Center of Veterinary and Comparative Oncology (CEROVEC), Genova, Italy.
Equine veterinary journal
|July 3, 2023
概括
马中的 Vulvar 状细胞癌 (VSCC) 与 Equus caballus 乳头瘤病毒 2 型 (EcPV2) 有关. 这项研究研究了EcPV2诱导的VSCC,并发现瘤中表皮细胞转移到介质细胞 (EMT) 的证据.
科学领域:
- 兽医瘤学 兽医瘤学
- 马类病理学 马类病理学
- 病毒性致癌发生.
背景情况:
- Vulvar状细胞癌 (VSCC) 是一种在马匹中被公认的疾病.
- 马匹瘤病毒2型 (EcPV2) 最近与VSCC的发展有关.
- 关于EcPV2诱导的VSCC存在有限的文献.
研究的目的:
- 为了记录一例自然发生的ecpv2诱导的vscc在马身上.
- 为了研究瘤对表皮细胞到介质细胞转换 (EMT) 的能力.
主要方法:
- 一份病例报告详细介绍了一头13岁的母马, vulva 质量快速增长.
- 组织病理学和分子分析,包括实时qPCR,RT-qPCR和RNAscope,以检测EcPV2及其蛋白表达 (E6/E7).
- 免疫组织化学 (IHC) 和RT-qPCR用于评估EMT标记物和基因表达.
主要成果:
- 在部病变中证实了ecPV2DNA和coprotein表达 (E6/E7).
- IHC表明了卡德林切换和EMT转录因子HIF1α的表达.
- 通过RT-qPCR检测发现EBI3,CDH2和CXCL8的显著上调,CDH1,IL12A和IL17的下调.
结论:
- 这些发现表明,在瘤性部病变中发生了表皮细胞到介质细胞转换 (EMT) 事件.
- 局限性包括无法概括发现,以及由于单个案例研究而导致过度解释的风险.
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