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相关概念视频

Growth of Cartilage and Bone Tissue01:27

Growth of Cartilage and Bone Tissue

3.4K
Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...
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Bone Formation by Endochondral Ossification01:24

Bone Formation by Endochondral Ossification

4.7K
Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...
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相关实验视频

Updated: Jul 24, 2025

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation
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超塑性人类巨质软骨用于关节再生

Ya Wen1,2,3, Yishan Chen1,2,3, Weiliang Wu4

  • 1Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, and Department of Orthopedic Surgery of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|July 3, 2023
PubMed
概括

这项研究开发了一种可扩展的人类宏软骨组织工程策略,使用一种新的定制培养基. 这种方法增强了软骨细胞的扩张和分化,产生了大型的状软骨移植,用于关节修复.

关键词:
细胞的可塑性 细胞的可塑性定制的扩展扩展关节再生 关节再生大质量软骨软骨.

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相关实验视频

Last Updated: Jul 24, 2025

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A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid
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科学领域:

  • 生物医学工程 生物医学工程
  • 再生医学是一种再生医学.
  • 组织工程是组织工程.

背景情况:

  • 软骨损伤是全球普遍存在的健康问题,需要有效的组织修复策略.
  • 目前的组织工程方法很难产生足够的软骨移植,以保持尺寸和表型.
  • 工程软骨在保持体特征的同时无法增长大小,这限制了其临床适用性.

研究的目的:

  • 为制造可扩展的人类宏大软骨组织制定一种新的逐步策略.
  • 增强人类软骨细胞的扩张和分化能力,以修复软骨.
  • 为了创建大尺寸的,现成的软骨类似物,具有类似氨酸的特性.

主要方法:

  • 利用了人类多节节性肌细胞和一个选定义的,无血清的定制培养 (CC) 介质.
  • 采用3D培养系统来促进宏软骨组织的形成.
  • 进行了转录基因分析,以阐明细胞增殖和分化的潜在细胞机制.
  • 在动物模型中对软骨缺陷进行了工程化宏软骨的体内疗效评估.

主要成果:

  • CC诱导的红细胞表现出14.59倍的扩张,同时保持了红细胞生成潜力.
  • 制造的宏软骨组织的平均直径为3.25 ± 0.05毫米,具有均的基质和完整的结构.
  • 与标准培养相比,细胞产量增加了2.57倍,II型原体表达增加了4.70倍.
  • 在体内研究表明,CC宏软骨保持了类的表型,并促进了显著的软骨缺陷愈合.

结论:

  • 开发的逐步策略使人类宏大软骨能够高效扩张,具有卓越的再生可塑性.
  • 这种方法克服了当前方法的局限性,允许同时增加尺寸和维护表型.
  • 工程化宏大软骨显示出显著的希望,作为一种现成的解决方案,用于关节再生和软骨修复.