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细胞外核糖核酸对骨关节炎中神经血管化的影响

Wen-Pin Qin^1,2, Qian-Qian Wan², Jian-Fei Yan^1,2

¹Department of Stomatology, Tangdu hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710032, P. R. China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|July 3, 2023

概括

骨关节炎关节中的细胞外RNA (exRNA) 与VEGF结合,促进神经和血管的生长. 针对这种RNA-VEGF复合体可能会提供新的关节炎治疗策略.

科学领域:

生物化学生化学
分子生物学分子生物学
整形外科整形外科整形外科

背景情况:

骨关节炎 (OA) 涉及到骨髓节结处的异常神经血管化.
与OA相关的神经血管化的调节机制尚不清楚.

研究的目的:

研究细胞外RNA (exRNA) 在OA相关的神经血管化中的作用.
探索OA中的exRNA和血管内皮生长因子 (VEGF) 之间的相互作用.

主要方法:

利用一种具有增强神经血管化的小鼠OA模型.
进行了体外结合试验和分子对接,以研究RNA-VEGF相互作用.
评估了RNA-VEGF复合体对内皮原生细胞和三腺细胞的影响.
研究了VEGF抑制剂和RNase治疗在体外和体外的影响.

主要成果:

增加的exRNA含量与神经血管化和OA关节中的VEGF表达相关.
通过静电相互作用与VEGF结合的合成RNA,形成RNA-VEGF复合体.
该RNA-VEGF复合体增强了内皮原生细胞和三角干细胞活动.
在体内抑制VEGF/VEGFR2或破坏RNA-VEGF复合体可以减少神经血管化和OA进展.

结论:

细胞外RNA (exRNA) 在调节骨关节炎中神经血管化的过程中起着重要作用.
RNA-VEGF复合体是OA中神经和血管内生长的关键媒介.
exRNAs代表了管理病态关节神经血管化的潜在治疗点.

关键词:

细胞外RNARNA是什么神经血管化的神经血管.骨关节炎是一种关节炎.骨质突节的结节.血管内皮生长因子血管内皮生长因子

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