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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

73
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
73
CRISPR01:59

CRISPR

52.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.4K

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相关实验视频

Updated: Jul 24, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

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通过CRISPRRNA架构的同时多功能转录组工程.

Zukai Liu1,2, Nathaniel Jillette1, Paul Robson1,2,3,4

  • 1The Jackson Laboratory for Genomic Medicine, Farmington, CT 06032, USA.

Nucleic acids research
|July 3, 2023
PubMed
概括
此摘要是机器生成的。

一个名为Combinatorial RNA Engineering via Scaffold Tagged gRNA (CREST) 的新平台可以同时操纵多个RNA目标. 这种系统显著减少了非目标效应,提高了RNA工程能力.

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CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
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CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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相关实验视频

Last Updated: Jul 24, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

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CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
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CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
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A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

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科学领域:

  • 分子生物学分子生物学
  • 在RNA生物学,RNA生物学.
  • 生物技术是生物技术.

背景情况:

  • 精确调节RNA处理和新陈代谢对于细胞完整性和功能至关重要.
  • 克里斯普尔-Cas13系统允许有针对性的RNA工程,但同时调制多个RNA处理步骤和非目标效应仍然是挑战.

研究的目的:

  • 开发一种用于同时,多功能RNA调制的新平台.
  • 克服现有的RNA工程工具的局限性,包括非目标事件.

主要方法:

  • 通过Scaffold开发了组合RNA工程 Tagged gRNA (CREST) 平台.
  • 将附加的RNA支架连接到Cas13 gRNA和与酶域结合的RNA结合蛋白.
  • 创建了用于RNA替代拼接和基编辑的双功能和三功能CREST系统 (A-to-G,C-to-U).
  • 使用分裂设计方法进行酶复制,以最大限度地减少目标之外的事件.

主要成果:

  • 证明了替代拼接和基基编辑的同时RNA操纵.
  • 使用分裂酶设计,实现了非目标事件的显著减少 (近99%).
  • 在目标部位成功复制了酶活性.

结论:

  • CREST平台可以同时执行多个RNA调制函数在不同的RNA目标上.
  • 分拆设计策略有效地减少了目标外影响,提高了特异性.
  • CREST扩展了用于RNA生物学研究的转录组工程工具箱.