对微生物殖民地计数自动化系统的评估
Elisa Heuser1, Karsten Becker1, Evgeny A Idelevich1,2
1Friedrich Loeffler-Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.
Microbiology spectrum
|July 3, 2023
概括
自动化殖民地计数系统在没有视觉校正的情况下显示的准确性很低,特别是在极端殖民地数量的板上. 手动校正提高了准确性,但对于微生物殖民地计数没有节省时间.
科学领域:
- 微生物学 微生物学
- 医学诊断 医学诊断 医学诊断
- 生物技术是生物技术.
背景情况:
- 微生物殖民地计数是研究和诊断的基础.
- 自动化系统旨在提高这种手动过程的效率.
- 评估自动化殖民地计数器的可靠性对于其采用至关重要.
研究的目的:
- 评估自动化殖民地计数仪器的准确性和时间效率.
- 为了比较自动计数与手动计数方法.
- 为了确定视觉校正对自动计数性能的影响.
主要方法:
- 评估了UVP ColonyDoc-It成像站的准确性和时间节省.
- 使用的标准化悬浮体包括金黄色葡萄球菌,大肠杆菌,Pseudomonas aeruginosa,Klebsiella pneumoniae,Enterococcus faecium和Candida albicans等.
- 盘子被手动计数,自动计数和视觉校正后自动计数.
主要成果:
- 完全自动计数显示,与手动计数的平均差异为59.7% (R2=0.77).
- 视觉校正将平均差异降低到1.8% (R2=0.99),实现了高一致性.
- 没有纠正的自动计数比手动 (70秒) 快 (30秒),但有了纠正,它更慢 (104秒).
结论:
- 完全自动的殖民地计数缺乏准确性,特别是在殖民地密度非常高或很低的情况下.
- 视觉校正显著提高了准确性,匹配手动计数.
- 与视觉校正自动计数殖民地并没有比手动计数提供时间优势.
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