探索L-异氨酸核糖开关,以增强Corynebacterium glutamicum中的4-氧异氨酸生产
Youhe Xiang1,2, Rui Chen1,2, Feng Shi3,4,5
1State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
Biotechnology letters
|July 3, 2023
概括
我们开发了一种L-Isoleucine (Ile) 诱导的生物传感器,以增强Corynebacterium glutamicum中的4-hydroxyisoleucine (4-HIL) 生产. 这种生物传感器有效下调Ile合成途径,从而增加4-HIL产量.
科学领域:
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
- 微生物生物技术 微生物生物技术
背景情况:
- L-单氨酸 (Ile) 生物合成是微生物中的一个复杂的途径.
- 4-基异氨酸 (4-HIL) 是一种有价值的化合物,具有多种应用.
- 优化4-HIL的微生物生产需要精确控制代谢途径.
研究的目的:
- 设计一种L-异黄素 (Ile) 诱导的生物传感器,用于动态调节Ile合成途径.
- 为了增强Corynebacterium glutamicum SN01.01中4-氧硫氨酸 (4-HIL) 的产生.
- 选和表征Ile-响应的核糖开关用于代谢控制.
主要方法:
- 基于TPP核糖开关的Ile诱导核糖开关 (IleRSN) 库的选.
- 在Corynebacterium glutamicum SN01.01中,ilvA基因上游的IleRSN的整合.
- 具有IleRS3-ilvA双复制集成和下调L-lysine生物合成的工程菌株.
主要成果:
- 四个Ile诱导的核糖转换 (IleRSN) 进行了选,IleRS1和IleRS3显示了与对照菌株相比的4-HIL标位.
- 在一个改性菌株 (KIRSA-3-9I) 中,IleRS3-ilvA的双复制集成显著增加了4-HIL的产生.
- 工程菌株KIRSA-3-9I获得了22.46 ± 0.96 g L-1的最终4-HIL标位,同时保持Ile水平低于35 mmol L-1.1.
结论:
- 开发的Ile诱导的核糖开关 (IleRS) 有效地对Corynebacterium glutamicum中的Ile合成途径进行动态下调.
- 选的IleRSN变种提供可调节的控制,可以在各种发酵条件下应用.
- 这一战略为增强微生物生产像4-HIL.这样有价值的化合物提供了一个强大的平台.
关键词:
4 - - 氧化硫氨酸.双重遗传选择是一种双重的遗传选择.动态调节 动态调节在L-Isoleucine生物传感器上.在Riboswitch中使用.在Riboswitch库中使用Riboswitch.更多相关视频
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