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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
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在Streptococcus anginosus中CRISPR-Cas II-A型系统的多样性
Richard Bauer1, Dorina Haider1, Aline Grempels1
1Institute of Medical Microbiology and Hygiene, University Medical Center, Ulm, Germany.
Frontiers in microbiology
|July 3, 2023
概括
这项研究揭示了Streptococcus anginosus具有CRISPR-CasII-A型系统,即使是以前与II-C型识别的系统. 对Streptococcus anginosus CRISPR位点的分析确定了两个不同的csn2基因,表明II-A型系统的变异.
科学领域:
- 微生物学 微生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 性链球菌 (Streptococcus anginosus) 是一种与侵袭性感染相关的共生细菌.
- 它的分子遗传背景,特别是CRISPR-Cas系统,尚不清楚.
- 在S. anginosus中报告了CRISPR-Cas类型II-A和II-C系统.
研究的目的:
- 在Streptococcus物种中对CRISPR-Cas II型系统的Cas9序列进行基因基因分析,重点是S. anginosus.
- 调查S. anginosus中的CRISPR-Cas II型系统的遗传基础.
- 鉴定S. anginosus中的CRISPR位点和相关的csn2基因的特征.
主要方法:
- 来自CRISPR-Cas II型系统的Cas9序列的家族遗传学分析.
- 使用家政基因对S. anginosus菌株进行多部位序列类型化 (MLST) 分析.
- 对CRISPR位点的分析和在S. anginosus中检测csn2基因变异的检测.
主要成果:
- 所有分析的S. anginosus Cas9序列与CRISPR II-A型系统集群,包括以前被指定为II-C型的系统.
- 来自其他细菌物种CRISPR-Cas II-C型系统的Cas9基因形成了一个独特的集群.
- 在S. anginosus CRISPR loci中发现了两个不同的csn2基因变异:一个类似于S. pyogenes的短形式和类似于Streptococcus thermophilus的长形式.
结论:
- 性链球菌 (Streptococcus anginosus) 似乎主要存在CRISPR-Cas II-A型系统的一个变异.
- 在II-C型系统报告的菌株中存在长 csn2 变异,这表明一种修改后的II-A型系统,而不是一个独特的II-C型系统.
- 进一步描述S. anginosus中的CRISPR-Cas系统对于了解其遗传构成和在感染中的作用至关重要.
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