HSV-1选择性地将转录因子Oct-1包装到EV中,以促进其感染
Yilei Ma1, Xiaomei Deng1, Lingyue Zhou1
1The Centre for Infection and Immunity Studies, School of Medicine, Sun Yat-sen University, Shenzhen, Guangdong, China.
Frontiers in microbiology
|July 3, 2023
概括
简单疹病毒1型 (HSV-1) 劫持细胞外囊泡 (EVs),以输出亲病毒蛋白Octamer-binding转录因子-1 (Oct-1),增强病毒基因转录和传播. 这项研究揭示了HSV-1感染传播的新机制.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 简单疹病毒1型 (HSV-1) 在其生命周期中利用细胞机制,包括对细胞外囊泡 (EVs) 的操纵.
- 以前的研究表明,HSV-1感染细胞的EV可以含有宿主限制因子,这表明它们在病毒感染中起着复杂的作用.
- 在HSV-1感染期间,这些EV内的特定宿主蛋白的功能仍然不完全理解.
研究的目的:
- 研究八体结合转录因子-1 (Oct-1) 在HSV-1相关的细胞外囊中作为载荷的作用.
- 为了确定EV介导的Oct-1转移是否有助于随后的HSV-1感染.
- 探索EV货物对病毒传播和宿主病原体相互作用的更广泛影响.
主要方法:
- 免疫光显微镜用于追踪感染细胞中的Oct-1局部化和与病毒蛋白 (VP16) 的同位化.
- 从HSV-1感染细胞中分析EV含量,特别是检查Oct-1和HCF-1.
- 使用在Oct-1淘汰赛 (KO) 细胞中培养的HSV-1进行感染测定,以评估病毒基因转录和传染性.
- 在接受细胞中吸收和核进口EV相关的Oct-1的研究.
- 试验旨在评估HSV-1感染细胞的EVs对随后感染囊泡性口腔炎病毒 (VSV) 的影响.
主要成果:
- 核转录因子Oct-1在HSV-1感染期间被发现在细胞外囊中分泌,经常与VP16同位.
- 在Oct-1 KO细胞中培养的HSV-1在随后的感染周期中显著减少了病毒基因转录.
- HSV-1积极促进了Oct-1的出口到不含virion的EV中,但不是HCF-1.
- 与EV相关的Oct-1被有效地导入受体细胞的细胞核,促进HSV-1感染.
- 来自HSV-1感染细胞的EV也将宿主细胞用于感染囊泡性口腔炎病毒.
结论:
- 在HSV-1感染期间,Oct-1被确定为包装在细胞外囊中的亲病毒宿主蛋白,有助于病毒传播.
- 该研究强调了EVs在运输功能宿主因子方面的作用,以促进病毒复制和传播.
- HSV-1利用囊泡分泌途径提供Oct-1,强调了涉及EVs的病毒策略的复杂性和异质性.
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