含有 metionin 的细胞内和细胞外蛋白质的抗氧化作用
Mark A Rosenfeld1, Lyubov V Yurina1, Alexandra D Vasilyeva1
1N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334 Russia.
Biophysical reviews
|July 3, 2023
概括
蛋白质中的甲氨酸氧化可以作为抗氧化剂对反应性氧物种 (ROS) 的防御. 然而,其在血中的不可逆转性质引发了关于其对细胞外蛋白的保护作用的问题.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 氧化压力研究研究 氧化压力研究
背景情况:
- 蛋白质中的氨酸残留物可以经历可逆氧化,作为对反应性氧物种 (ROS) 的保护机制.
- 在血蛋白等细胞外蛋白质中甲氨酸的氧化在很大程度上是不可逆转的,因为血中没有甲氨酸硫氧化减少酶 (MSR).
- 由于其不可逆转性和对蛋白质结构和功能的潜在影响,在血蛋白中甲酸氧化的功能影响仍在争论中.
研究的目的:
- 对细胞内和细胞外蛋白质的氧化修饰的现有数据进行审查.
- 研究蛋白质中氨酸残留物的抗氧化作用,特别是它们在氧化应激下对蛋白质结构和功能的影响.
- 强调需要进一步研究血蛋白中氨酸氧化的体内后果及其在恒温和ROS相关疾病中的作用.
主要方法:
- 对蛋白质氧化修饰研究的文献综述.
- 从体外和体内实验中分析有关氨酸氧化的数据.
- 在细胞内与细胞外蛋白质中氧化修饰的比较评估.
主要成果:
- 细胞内和细胞外的蛋白质都含有可以作为抗氧化剂的氨酸残留物.
- 氧化某些氨酸残留物,特别是表面暴露的残留物,可能不会显著损害蛋白质功能.
- 有证据表明,表面暴露的甲因在减轻氧化损伤方面具有潜在的抗氧化作用.
结论:
- 甲氨酸氧化在蛋白质抗氧化防御中起作用,但其功能后果因蛋白质的位置和结构而异.
- 血蛋白中氨酸氧化不可逆转的性质需要进一步研究它们的长期结构和功能完整性.
- 了解甲氨酸氧化对于阐明氧化应激下蛋白质结构-功能关系及其对ROS相关病理的影响至关重要.
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