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提取一些必不可少的氨基酸,使用由基于糖的深溶解剂制成的水性双相系统

İnci Söğütlü¹, Shakir Mahmood Saeed², Mohaned Adil³

¹Republic of Turkey Ministry of Agriculture and Forestry Turkey.

|July 3, 2023

概括

新型的深溶解溶剂形成水性双相系统 (ATPS) 以有效提取氨基酸. 这些系统对生物技术和制药应用具有前景.

科学领域:

生物化学生物化学
分离科学分离科学
绿色化学绿色化学

背景情况:

为生物分子提取建立了水性双相系统 (ATPS).
深度欧性溶剂 (DES) 为形成ATPS提供了一种新的方法.

研究的目的:

为了确定由聚乙烯糖醇二甲基乙太250和两个特定的天然深度溶解剂 (NADES) 形成的ATPS的相图.
为了评估这些ATPS对特定氨基酸的提取效率.

主要方法:

阶段图的测定使用结线数据对聚乙烯基醇二甲基乙醇250与胆化物/糖或胆化物/果糖NADESs.
将双节数据与默丘克和扎法拉尼-莫塔尔方程相匹配.
氨基酸提取 (l-氨酸,l-氨酸,l-氨酸) 和使用钻石-Hsu方程的分区系数相关性.

主要成果:

基于NADES的ATPS由于不完全破坏键而表现出三元状的行为.
通过使用已建立的实证方程,成功地将二极管数据配合在一起.
能够有效地提取l-氨酸,l-氨酸和l-氨酸.

结论:

可以有效地利用NADES来创建用于生物分子分离的新型ATPS.
这些系统显示出在生物技术过程中有效提取氨基酸的潜力.
预计将在制药和相关领域进一步应用.

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