基因SH3BGRL3通过基于竞争性内源性RNA机制的circRNA_0010984调节急性髓性白血病的进展
Xiancong Yang1,2, Yaoyao Wang3, Simin Rong2
1Department of Clinical Laboratory, The Second Medical College of Binzhou Medical University, Yantai, China.
Frontiers in cell and developmental biology
|July 3, 2023
概括
循环RNAcircSH3BGRL3通过菌miR-375,增加YAP1表达,并激活Hippo通路来促进急性髓性白血病 (AML) 细胞增殖. 向circSH3BGRL3可能为AML治疗提供一种新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因规则 基因规则
背景情况:
- 急性髓性白血病 (AML) 是一种具有不良预后的血液性恶性瘤.
- 循环RNAs (circRNAs) 越来越多地被认为是它们在癌症发展中的作用.
- 从SH3BGRL3衍生出的circRNA_0010984在AML中的特定作用仍未得到充分研究.
研究的目的:
- 研究SH3BGRL3及其衍生的circRNA,circRNA_0010984在急性髓性白血病中的作用.
- 阐明circRNA_0010984在AML扩散中的作用背后的分子机制.
- 探索circRNA_0010984作为AML治疗点的潜力.
主要方法:
- 在AML中分析SH3BGRL3和circRNA_0010984的基因表达,使用qRT-PCR.
- 在体外细胞实验包括转染,增殖试验,细胞周期分析和分化.
- 使用RNA免疫沉和双露西法酶记者分析验证circRNA-miRNA相互作用 (circRNA_0010984和miR-375).
- 生物信息分析包括PPI网络建设和功能丰富 (GO,KEGG).
主要成果:
- circRNA_0010984在AML中显著上调,促进癌细胞增殖和阻止细胞循环.
- 抑制circRNA_0010984抑制了AML细胞增殖和诱导细胞循环停止.
- circRNA_0010984 作为miR-375的分子海绵,抑制其活性,并对其目标基因YAP1进行上调,从而激活Hippo信号通路.
结论:
- circRNA_0010984 在急性髓性白血病中起着关键的致癌作用.
- 该circSH3BGRL3/circRNA_0010984 - miR-375 - YAP1轴是驱动AML进展的关键途径.
- circRNA_0010984代表了AML潜在的诊断生物标志物和治疗点.
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