海马多细胞聚合物的新兴结构和功能性质
Victor P Acero1,2,3, Suradip Das1,2, Olivia Rivellini1,2
1Center for Brain Injury and Repair, Department of Neurosurgery, Perelman School of Medicine University of Pennsylvania, Philadelphia, PA, United States.
Frontiers in neuroscience
|July 3, 2023
概括
三维海马神经聚合物,与二维培养不同,促进生物忠诚网络特性. 这些3D模型为研究复杂的大脑功能和开发模块化神经网络拓学提供了一个有前途的平台.
科学领域:
- 神经科学是一个神经科学.
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
背景情况:
- 传统的二维神经元培养缺乏关键的大脑微环境元素.
- 二维文化在重建复杂的整合性网络属性方面是有限的.
- 存在对神经网络更生物忠实体内模型的需求.
研究的目的:
- 使用强迫聚合技术开发和描述3D海马神经聚合物.
- 将3D聚合物的结构和功能特性与传统的2D文化进行比较.
- 评估3D聚合物的潜力,作为复杂神经网络拓学的构建块.
主要方法:
- 从动物胚胎组织生成高密度的3D海马聚合物.
- 在体外 (DIV) 培养和分析3D聚合物和2D分离培养28天.
- 使用多电极阵列 (MEAs) 记录自发电生理活动.
主要成果:
- 3D聚合物比2D培养物更早地表现出增强的轴突结和神经元极化.
- 星细胞在3D培养中自我组织,并在类似于活体的形态学中发展.
- 3D网络显示了同步爆发活动和28DIV的高爆发率.
- 双聚合网络在7个DIV时开始活跃,单聚合网络在14个DIV时开始活跃.
结论:
- 海马聚合物的3D微环境支持生物忠实形态和功能性质.
- 神经聚合物可以回顾海马网络的新兴特性.
- 聚合物作为构建复杂多节点神经网络拓学的模块化构建块.
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