基于频谱效应关系分析,确定了Zishen Yutai药丸的潜在质量标志物
Sijia Wu1, Qiuling Huang2, Feiya Sheng3
1State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China.
Frontiers in pharmacology
|July 3, 2023
概括
这项研究确定了Zishen Yutai药丸 (ZYP) 中与其治疗作用相关的39个潜在的质量标志物. 这些发现为ZYP提供了科学依据.
科学领域:
- 传统中国医学 (TCM) 质量控制
- 药理学 药理学 是一个学科.
- 分析化学 分析化学
背景情况:
- 传统中医药 (TCM) 的质量评估由于复杂性而具有挑战性.
- 齐申玉泰药 (ZYP) 用于预防流产,但缺乏明确的质量标记和对其治疗基础的理解.
- 目前对ZYP的质量控制方法不足.
研究的目的:
- 确定ZYP与其药用活动相关的质量标志物.
- 建立一个科学质量控制和ZYP的产品改进的基础.
- 阐明ZYP有效性的化学成分和药理基础.
主要方法:
- 使用2D-LC-LTQ-Orbitrap-MS.进行ZYP的全面化学成分分析.
- 在体外 (HTR-8/SVneo模型) 和体内 (小鼠模型) 评估27个ZYP直角组的疗效.
- 频谱效应关系分析,以将化学成分与药理活动联系起来.
主要成果:
- 在ZYP中确定了589种化学成分,其中139种以前没有报告.
- 通过直角设计和频谱效应分析成功确定了潜在的质量标记.
- 根据质谱和药理学数据,确定了39种物质作为潜在的质量标志物.
结论:
- 该研究提供了一种可行的策略,用于发现TCM中的生物活性质量标记物.
- 鉴定到的标记物为改善质量控制和对ZYP治疗效果的理解提供了基础.
- 这种方法可以推进复杂的传统中医药配方的质量评估.
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