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相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.9K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.9K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: Jul 24, 2025

Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation
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Author Spotlight: Advancements in Correlative Light and Electron Microscopy with Fluorescent Protein Preservation

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DeepCLEM:使用深度学习对相关光和电子显微镜进行自动注册.

Rick Seifert1,2, Sebastian M Markert2, Sebastian Britz2

  • 1Center for Computational and Theoretical Biology, University of Würzburg, Würzburg, 97074, Germany.

F1000Research
|July 3, 2023
PubMed
概括

DeepCLEM通过从EM图像中预测光度来自动化相关光和电子显微镜 (CLEM) 注册. 这种新的方法消除了手动对齐,提高了生物成像工作流程的精度.

关键词:
相对显微镜学 相对显微镜学深度学习 (Deep Learning) 是一种深度学习.图像注册 图像注册 图像注册在-silico标签的标签.

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Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
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Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

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相关实验视频

Last Updated: Jul 24, 2025

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科学领域:

  • 显微镜的使用方法
  • 生物技术是生物技术.
  • 计算生物学 计算生物学

背景情况:

  • 相对光和电子显微镜 (CLEM) 需要在光和电子显微镜 (EM) 数据集之间进行精确的图像注册.
  • 目前的注册方法往往是手动或半自动的,依赖光斑或信托标记,这可能耗时并引入不准确性.
  • 光和EM模式之间的图像对比差异阻碍了直接的自动对齐.

研究的目的:

  • 在CLEM中开发一个完全自动化的工作流程,用于精确的图像注册.
  • 克服手动和半自动注册方法在CLEM中的局限性.
  • 为了提供一个强大的和适应性的解决方案,用于对比成像数据.

主要方法:

  • 介绍了DeepCLEM,这是一个全新的,完全自动化的CLEM注册工作流程.
  • 使用卷积神经网络 (CNN) 来直接从EM图像中预测光信号.
  • 使用基于关联的对齐来记录预测的光信号与实验测量的染色体信号.

主要成果:

  • DeepCLEM实现了光和EM图像的全自动注册.
  • 该工作流准确地预测来自EM数据的光信号.
  • 使用基于相关性的对准与染色质信号来证明成功注册.

结论:

  • 在自动化CLEM图像注册方面,DeepCLEM提供了显著的进步.
  • 斐济插件促进了可访问性和集成到现有的显微镜工作流程.
  • 该方法具有适应其他成像模式和3D数据集的潜力.