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伪尤里丁指导生殖细菌小RNA传输和表观遗传遗传
Rowan P Herridge1, Jakub Dolata1,2, Valentina Migliori3,4
1Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, USA.
bioRxiv : the preprint server for biology
|July 3, 2023
概括
伪尤里丁 (Ψ),一种常见的RNA修饰,标志着植物和动物生殖系中遗传的小RNA. 这一发现揭示了跨物种表观遗传的保存机制.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- RNA生物学的RNA生物学
- 发育生物学是发展生物学.
背景情况:
- 表观遗传修饰指导发育,但通常在雌性体形成过程中被重置.
- 小RNAs参与表观遗传,但区分它们的机制尚不清楚.
- 伪尤里丁 (Ψ) 在RNA中丰富,但其在小RNA中的作用尚未被探索.
研究的目的:
- 为了研究伪尿素 (Ψ) 在小RNA中的存在和作用.
- 为了确定 Ψ 标志是否在植物和动物的生殖系中继承了小RNA.
主要方法:
- 在短RNA序列中检测伪乌里丁 (Ψ) 的新型检测方法的开发.
- 在小鼠和阿拉比多普西斯的微RNA和小RNA中检测 Ψ.
- 与PAUSED/HEN5 (PSD) 和Exportin-t.t.进行的遗传相互作用研究.
主要成果:
- 伪尤里丁 (Ψ) 存在于小鼠和阿拉比多普西斯的微RNA及其前体中.
- 胚胎小RNAs,包括Arabidopsis表观遗传激活siRNAs (easiRNAs) 和小鼠piwi相互作用RNAs,为 Ψ.
- 伪尿化easiRNAs局部化到精子细胞,并且需要PSD来运输它们.
- 导出-t对于三倍体块至关重要,这是一个表观遗传性遗传特征.
结论:
- 伪尤里丁 (Ψ) 是基因线中遗传小RNA的保存标记物.
- Ψ在植物和动物的表观遗传中起作用.
- PSD/Exportin-t通路对于将小RNA输送到精子细胞和确保适当的表观遗传至关重要.
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