相关实验视频

Updated: Jul 24, 2025

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

CRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans

Published on: November 14, 2018

扩大基因操纵和发现的工具包在Candida物种使用基于CRISPR核糖蛋白的方法

Justin B Gregor¹, Victor A Gutierrez-Schultz¹, Smriti Hoda¹

¹Department of Biochemistry.

bioRxiv : the preprint server for biology

|July 3, 2023

概括

这项研究通过引入新的耐药性磁带来扩大了Candida病原体的遗传工具,使得基因编辑更具多样性. 这推动了真菌生物学和抗真菌药物耐药性的研究.

科学领域:

微生物学微生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

世界卫生组织确定了优先的真菌病原体,包括Candida物种,需要先进的基因操纵工具.
目前在Candida中的基因操纵受到药物耐药性磁带的可用性限制,主要是NatMX和HphMX.
克里斯普尔-Cas9系统已经显示出希望,但需要扩展的遗传工具包,以更广泛的应用.

结论:

该CRISPR-Cas9 RNP系统有效地重新使用Saccharomyces cerevisiae遗传工具包用于Candida研究.
这个扩展的工具包为真菌生物学和抗真菌药物耐药性的机制提供了新的见解.
促进对优先的真菌病原体进行更全面的遗传研究,有助于开发新的治疗策略.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?在Candida albicans中使用.它们是Candida auris.在Candida glabrata的研究中.在KanMX和BleMX中.设置1 基因组 H3K4 甲基转移酶.药物耐药性的录音带.埃尔戈斯醇通道的路径菌病原体是一种真菌病原体.基因组编辑基因组编辑同质性定向维修指导维修核糖核蛋白粒子 (RNP) 是一种核糖核蛋白粒子.

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CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

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