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Updated: Jul 24, 2025

Chromatin Immunoprecipitation from Human Embryonic Stem Cells
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Chromatin Immunoprecipitation from Human Embryonic Stem Cells

Published on: July 22, 2008

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在人类胚胎干细胞中发现了有能力的染色体区域

Julian Pulecio, Zakieh Tayyebi, Dingyu Liu

    bioRxiv : the preprint server for biology
    |July 3, 2023
    PubMed
    概括

    人类胚胎干细胞 (ESCs) 具有预先建立的染色质区域 (CCRs),可以在分化过程中快速激活特定基因. 这些不同的调节区域保持多能性和细胞可塑性.

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    Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture 4C10:02

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    相关概念视频

    Chromatin Modification in iPS Cells01:32

    Chromatin Modification in iPS Cells

    Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
    Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
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    Embryonic Stem Cells00:57

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    Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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    The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
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