处理和储存方法影响口腔和肠道微生物组的组成
bioRxiv : the preprint server for biology
|July 3, 2023
概括
微生物组样本处理对微生物多样性产生影响. 解热化便,新鲜的唾液和牙上游物样本显示出最高的多样性,为一致的微生物组研究提供了关键的见解.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 微生物组研究依赖于16S rRNA基因测序进行微生物组成分析.
- 不一致的样本存储和处理方法可以引入变化并影响研究结果.
- 预分析方法的标准化对于可靠和可比的微生物组数据至关重要.
结论:
- 样品处理显著影响微生物群的多样性和组成.
- 建议对便样本进行冷化,对于口腔样本进行特定分量,以保持多样性.
- 标准化预分析工作流程对于减轻混因素和改善微生物组研究的跨研究可比性至关重要.
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