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IgM N-糖化与COVID-19的严重程度和补体沉积率相关
Benjamin Haslund-Gourley1, Kyra Woloszcuk1, Jintong Hou1
1Drexel University College of Medicine.
Research square
|July 3, 2023
概括
在COVID-19患者中调查免疫球蛋白M (IgM) N-甘化,发现与疾病严重程度相关的明显的甘氨基形状. 改变IgM的曼诺基化和化与临床标记物和细胞因子水平相关,影响免疫反应.
科学领域:
- 免疫学 免疫学 免疫学
- 葡萄糖生物学 葡萄糖生物学
- 病毒学 病毒学
背景情况:
- 免疫球蛋白G (IgG) 糖化在SARS-CoV-2感染中至关重要,但免疫球蛋白M (IgM) N-糖化在急性人类病毒性疾病中的作用仍然未被探索.
- 实验室研究表明,IgM糖化对T细胞增殖和补体激活有影响.
研究的目的:
- 为了研究不同COVID-19严重程度的患者IgM的N-糖化概况.
- 确定IgM N-糖化模式与COVID-19严重程度的临床指标之间的关联.
- 探索IgM糖化,细胞因子概况和免疫标记物之间的关系.
主要方法:
- 在健康对照组和住院COVID-19患者 (中度和重度) 中分析血清IgM N-糖化.
- 使用质谱法对IgM甘氨酸的曼诺基化和化水平进行量化.
- 糖甘特征,临床严重性标志物 (D-二聚体,BUN等) 之间的相关性分析. ),细胞因子水平 (IL-16,IL-18) 和抗体标位 (IgG,IgA,IgM).
- 检查外围血液单核细胞 (PBMC) mRNA转录的基因表达.
- 在IgM上识别特定的酸链接 (alpha-2,3和alpha-2,6).
主要成果:
- 与中度病例相比,严重的COVID-19患者在血清IgM上表现出增加的二和三化甘氨酸和改变的曼诺斯甘氨酸.
- 这些变化与在重症患者的IgG上观察到的酸水平下降形成鲜明对比.
- IgM的曼诺基化和化水平与疾病严重程度标志物和早期抗体反应有显著的相关性.
- IL-16和IL-18细胞因子的趋势反映了IgM糖化模式,这表明它们有调节作用.
- 在PBMC中戈尔吉曼诺酶的减少表达与IgM上曼诺处理的减少相关.
- 在严重的COVID-19中注意到抗原特异性IgM抗体依赖补充沉积的升高.
结论:
- IgM N-糖化模式与COVID-19的严重程度有关,与疾病进展相关的特定糖变化.
- 在严重的COVID-19中观察到高抗原特异性IgM抗体依赖补充沉积.
- 了解IgM糖化对病毒感染期间免疫功能的影响至关重要.
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