Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Regulated mRNA Transport02:22

Regulated mRNA Transport

6.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Principles of resident tissue macrophages revealed by the eye.

Advances in immunology·2026
Same author

Using Patient iPSC-derived Retinal Pigment Epithelial Cells to Evaluate Differential Susceptibility to MEK Inhibitor-Associated Retinopathy.

bioRxiv : the preprint server for biology·2026
Same author

A Porcine Model of Rhegmatogenous Retinal Detachment and Repair With Pars Plana Vitrectomy and Silicone Oil Tamponade.

Investigative ophthalmology & visual science·2026
Same author

Single-Cell Gene Expression and eQTL Analyses in the Human Retina, RPE, and Choroid in Macular Degeneration.

bioRxiv : the preprint server for biology·2026
Same author

Metabolic Analysis of Human Retinal Pigment Epithelium and Choroid Tissue in Aging and Macular Degeneration.

bioRxiv : the preprint server for biology·2026
Same author

Acriflavine delivery via Polyurethane nanocapsules to treat neovascular age-related macular degeneration.

bioRxiv : the preprint server for biology·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jul 24, 2025

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
09:45

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Published on: March 14, 2022

3.0K

基因表达在人类冠状动脉神经血管膜内使用空间转录学.

Andrew P Voigt1,2, Nathaniel K Mullin1,2, Emma M Navratil1,2

  • 1Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242.

bioRxiv : the preprint server for biology
|July 3, 2023
PubMed
概括
此摘要是机器生成的。

这项研究使用空间RNA测序来分析黄斑新血管化中的基因表达,识别内皮细胞中的失调基因和潜在的上游调节者,如VEGF和TGFB1.

关键词:
在这个过程中,AMD是AMD.胆体 胆体 胆体基因表达的基因表达方式新血管化的新血管化.空间RNA序列的测序方法

更多相关视频

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K
Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.7K

相关实验视频

Last Updated: Jul 24, 2025

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
09:45

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Published on: March 14, 2022

3.0K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K
Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.7K

科学领域:

  • 眼科医生 眼科 眼科
  • 分子生物学分子生物学
  • 基因组学就是基因组学.

背景情况:

  • 黄斑新血管化 (MNV) 是与年龄相关的黄斑退化的一个关键并发症,导致视力丧失.
  • 推动MNV病变的细胞起源和分子机制仍然不完全理解.
  • 在MNV中,病理性血管新生可以来自胸腔或视网膜来源.

研究的目的:

  • 在人类斑点新血管化中空间描述基因表达.
  • 在MNV中识别失调的基因及其细胞类型起源.
  • 为了发现MNV的潜在分子驱动因素.

主要方法:

  • 用MNV和健康对照对人类捐赠眼睛的空间RNA测序.
  • 生物信息分析,包括解卷算法,以预测细胞类型特定的基因表达.
  • 空间概况与现有的单细胞RNA测序数据和小鼠模型的比较.

主要成果:

  • 在MNV区域中丰富的鉴定基因.
  • 预测Rho家族GTPase和整体素信号基因在MNV内的内皮细胞中的表达增加.
  • 鉴定出VEGF和TGFB1是内皮和视网膜色素上皮细胞中基因表达变化的潜在上游调节者.

结论:

  • 空间转录组学提供了对MNV细胞失调的见解.
  • 确定了候选基因和通路,可能为MNV提供治疗点.
  • 在眼组织中确认已知的区域基因表达模式.