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在两个不同的扩展器中评估马精子宏:与PNA/PSA/PI三重染色相比,Spermac染色

H Dierberger¹, L Pieper¹, M Jung¹

¹Institute for Reproduction of Farm Animals Schönow, Bernau, Germany.

Reproduction in domestic animals = Zuchthygiene

|July 3, 2023

概括

评估马宏需要专门的染料. 使用PNA/PSA/PI三重染色的流细胞计显示出比Spermac染色更好的一致性,特别是在Gent扩展器中,这表明适应的协议对于准确的结果至关重要.

科学领域:

动物繁殖动物繁殖
精液分析精液分析
流动细胞计量 (Flow Cytometry) 是一种流动细胞计.

背景情况:

马的青体很小,如果没有专门的染色技术,很难评估.
准确评估体完整性对于子生育能力的评估至关重要.

研究的目的:

为了比较Spermac染色和PNA/PSA/PI三重染色的方法协议,通过流动细胞计测量检测非完整的马青.
通过两种不同的精液扩展器来评估这些方法:EquiPlus和Gent.

主要方法:

18个马的射精被分割和稀释在EquiPlus或Gent扩展器中.
精液样本使用Spermac染色和PNA/PSA/PI三重染色进行染色.
在采集后4至240小时内,使用流式细胞计评估了体完整性.

主要成果:

在EquiPlus扩展器中的方法之间有很好的相关性 (r=0.77).
根特扩展器中的方法之间的公平相关性 (r=0.49).
流细胞测量检测到EquiPlus中的非完好无损的体比Gent更多 (p<0.001),而Spermac染色没有显示差异 (p=0.902).

结论:

通过流动细胞计测试进行PNA/PSA/PI三重染色,与Spermac染色相比,特别是在Gent扩展剂中,证明了优越的方法协议.
蛋黄在Gent扩展器中可能会导致人工制造物,阻碍Spermac污点的解释.
适应的实验室协议对于不同的精液扩展剂至关重要,以确保在马精子评估中获得可比和可靠的结果.

关键词:

一个宏体的宏体.扩展器扩展器扩展器流动细胞计量是流动细胞计量的方法.染色的染色方式马匹的精子是子的精子.

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