生物修饰的细胞外囊泡重塑肠道微环境以克服辐射肠炎
Hang Li1, Shuya Zhao1, Mian Jiang1
1Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, P.R. China.
ACS nano
|July 3, 2023
概括
来自被辐射的捐赠者的小细胞外囊泡 (外体) 能够防止辐射肠炎. 携带miRNA-142-5p的增强外体为胃肠道提供了更好的辐射保护.
科学领域:
- 细胞生物学 细胞生物学
- 辐射生物学 辐射生物学
- 纳米医学是一种纳米医学.
背景情况:
- 电离辐射 (IR) 引起肠炎,这是一个重大的临床挑战.
- 细胞外囊泡 (EVs) 调节组织微环境.
- 需要使用EV用于肠道损伤的辐射保护策略.
研究的目的:
- 研究小EVs (外体) 以防辐射,防止辐射引起的肠道损伤.
- 在外体内识别介导保护的功能分子.
- 加强基于电动汽车的辐射保护策略.
主要方法:
- 从被辐射的小鼠中分离出外体.
- 向接受者小鼠注射外体,以评估对全身辐射 (TBI) 的保护.
- 进行了外体微RNA (miRNA) 分析.
- 在肠道上皮细胞中研究了miRNA-142-5p的功能.
- EVs被生物修饰以增强miRNA-142的表达和向.
主要成果:
- 暴露于TBI的捐赠者的外体保护了接受者免受TBI致命性和GI毒性.
- miRNA-142-5p在被辐射的小鼠和放射治疗患者的外体中被上调.
- miR-142保护了肠道细胞免受辐射诱导的亡.
- 电动汽车改善了肠道微环境,调解了保护.
- 生物修饰的EV显示出对辐射肠炎的增强保护.
结论:
- 外体细胞介于对肠道损伤的辐射保护.
- miRNA-142-5p是外体介导辐射保护中的一个关键功能分子.
- 生物修饰外体为辐射诱导的胃肠综合征提供了一个有前途的治疗方法.
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