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细胞表面核蛋白表达:一种贝塔冠状病毒免疫调节策略
Alberto Domingo López-Muñoz1, Jefferson J S Santos1, Jonathan W Yewdell1
1Cellular Biology Section, Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases National Institutes of Health, Bethesda, MD 20892.
人类冠状病毒 (HCoV),包括SARS-CoV-2和普通感冒菌株,在细胞表面表达核囊体 (N) 蛋白. 这些N蛋白操纵免疫细胞的迁移和功能,影响先天免疫力.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- SARS-CoV-2核体 (N) 蛋白在细胞表面表达,激活免疫细胞并抑制白细胞化学反应.
- 这项研究调查了来自普通感冒的人类冠状病毒 (HCoV) -OC43.3的核体 (N) 蛋白的功能.
研究的目的:
- 将SARS-CoV-2 N蛋白的发现扩展到HCoV-OC43 N蛋白.
- 研究细胞表面HCoV N蛋白在天生的免疫和适应性免疫中的作用.
主要方法:
- 在受感染和未受感染的细胞上研究了HCoV-OC43 N蛋白质表达.
- 分析了 HCoV-OC43 N 蛋白与化学因子和细胞因子的结合.
- 进行化疗检测,以评估白细胞迁移抑制.
主要成果:
- HCoV-OC43 N 蛋白通过结合肝素硫酸盐/肝素,在细胞表面表达.
- HCoV-OC43 N蛋白与11种人类化学因子和6种细胞因子结合.
- HCoV-OC43 N蛋白质抑制白细胞迁移,类似于其他致病性和普通感冒 HCoV N 蛋白质.
结论:
- 细胞表面 HCoV N 蛋白质在操纵宿主天生的免疫力方面发挥着保守的作用.
- HCoV N 蛋白质是适应性免疫的潜在目标.
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