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与多重标记的细菌突变体配对的单细胞宿主概况揭示了细胞内毒性-免疫网络

Ori Heyman¹, Dror Yehezkel¹, Camilla Ciolli Mattioli¹

¹Department of Immunology and Regenerative Biology, Weizmann Institute of Science, Rehovot 7610001, Israel.

Proceedings of the National Academy of Sciences of the United States of America

|July 3, 2023

概括

我们开发了scPAIR-seq,这是一种新的单细胞方法,用于分析细菌毒性因素和感染期间宿主免疫反应. 这种方法揭示了细菌突变物如何影响宿主细胞,进步了我们对感染动态的理解.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
基因组学就是基因组学.

背景情况:

主体-病原体相互作用驱动复杂的感染结果.
单细胞RNA测序 (scRNA-seq) 主要分析宿主反应,对细菌因子分析的能力有限.
了解细菌毒性机制对于对抗传染病至关重要.

研究的目的:

开发一种单细胞方法 (scPAIR-seq) 用于同时分析宿主转录组和细菌突变条形码.
功能性地评估细菌突变对宿主细胞表型的影响.
绘制细菌效应者的毒性网络,以及它们与宿主免疫路径的相互作用.

主要方法:

scPAIR-seq:一种单细胞方法,将scRNA-seq与聚合的条形码细菌突变库相结合.
宿主细胞 (巨细胞) 感染一个 * Salmonella * Typhimurium 分泌系统效应因子突变的库.
通过scRNA-seq.捕获的宿主转录组变化和细菌突变条形码的分析.

主要成果:

scPAIR-seq成功捕获了宿主细胞转录组和细菌突变条形码.
确定了单个细菌突变的效应器冗余和独特的功能指纹.
根据它们对宿主免疫路径的影响,绘制了Salmonella*效应者的全球毒性网络.

结论:

scPAIR-seq是一个强大的工具,用于剖析单细胞水平的细菌毒性策略.
该方法能够全面分析宿主-病原体相互作用和免疫逃避机制.
提供了关于导致感染结果的复杂相互作用的见解.

关键词:

沙门氏菌是一种沙门氏菌.主体病原体相互作用巨细胞是什么?巨细胞是什么?一个单细胞RNA测序.

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