NLRs消除了MED10b和MED7介导的斯蒙酸依赖转录的抑制,以激活免疫力
Qian Wu1, Cong Tong1, Zhengqiang Chen1
1The Key Laboratory of Plant Immunity, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, P. R. China.
概括
植物免疫受体称为核酸结合域,富含白素的重复含有受体 (NLRs) 激活防御. 这项研究表明,MED10b和MED7蛋白抑制莉花体信号传递,NLRs调节它们以触发对病原体的免疫力.
科学领域:
- 植物免疫力 植物免疫力
- 分子植物病原体相互作用
- 基因调节 基因调节
背景情况:
- 植物细胞内核酸结合域,富含白素的重复含有受体 (NLR) 检测病原体效应物并触发免疫反应.
- 调解器复合体对于通过将转录因子与转录机械联系起来来调节基因转录至关重要.
- 了解NLR如何激活下游免疫基因对于植物防御至关重要.
研究的目的:
- 调查中介体复合体在NLR中介植物免疫中的作用.
- 阐明了线圈NLR (CNL) 激活防御信号通路的机制.
- 为了确定Solanaceae. jasmonate-dependent防御反应的新型调节者.
主要方法:
- 酵母两杂交试验和共同免疫沉以研究蛋白质相互作用.
- 基因淘汰和淘汰实验用于评估基因功能.
- 定量实时PCR用于分析基因表达.
- 对植物防御对托斯波病毒的反应的分析.
主要成果:
- 调解器复合体的MED10b和MED7子单元调解斯酸盐依赖的转录抑制.
- 番茄CNL Sw-5b的卷卷卷 (CC) 域与MED10b直接相互作用.
- 干扰MED10b或MED7会激活植物对托斯波病毒的防御,这表明它们在免疫力方面的压制性作用.
- MED10b与MED7相互作用,MED7反过来与JAZ蛋白相互作用,形成对桑酸 (JA) 敏感基因的抑制综合体.
- 激活的Sw-5b CC干扰了MED10b-MED7的相互作用,释放了压制并激活了JA-依赖的防御.
- 包括NRC在内的其他CNL的CC域也调节MED10b/MED7以激活对各种病原体的防御.
结论:
- MED10b和MED7作为莉花酸依赖转录的新型抑制剂.
- 在Solanaceae中,各种CNL利用MED10b/MED7模块来激活JA特定的防御通路.
- 这项研究揭示了NLR介导免疫的新机制,涉及调节介导体复合体和斯酸盐信号传递.
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