外源酸通过SAPK8-ABF1-Ehd1/Ehd2通路抑制了大米的开花
Liqun Tang1, Guanghao Li1, Huimei Wang1
1State key laboratory of rice biology and breeding, China National Rice Research Institute, Hangzhou 311400, China.
Journal of advanced research
|July 3, 2023
概括
这项研究揭示了ABA信号是如何通过SAPK8-ABF1通路延迟花的. 这种机制涉及关键的开花基因的表观遗传抑制,影响产量和适应性.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 米的开花时间对于产量和适应性至关重要.
- 酸 (ABA) 影响水开花,但其分子通路尚不清楚.
研究的目的:
- 为了阐明由ABA介导的米开花抑制的分子机制.
- 为了确定关键的基因和途径,涉及到ABA对开花时间的影响.
主要方法:
- 在CRISPR-Cas9基因编辑中创建突变 (abf1,sapk8).
- 生物化学试验 (酵母二混合,拉下,激酶试验) 来研究蛋白质相互作用和酸化.
- 分子技术 (ChIP-qPCR,EMSA,LUC测定) 用于分析基因结合和转录活性.
主要成果:
- 发现了一种新的途径,SAPK8-ABF1-Ehd1/Ehd2,它调解了ABA抑制开花的过程.
- SAPK8可化ABF1,增强其与Ehd1和Ehd2促进体的结合.
- ABF1招募了PRC2复合体,导致H3K27me3修饰和抑制了开花基因的转录,导致延迟开花.
结论:
- SAPK8和ABF1在ABA信号和花控制中发挥着至关重要的作用.
- 一个PRC2介导的表观遗传抑制机制是ABF1调节ABA诱导的花期延迟的基础.
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