在Aspergillus niger中β-ketoadipate通路的protocatechuate分支的功能分析
Michael Sgro1, Nicholas Chow2, Farnaz Olyaei2
1Department of Biology, Concordia University, Montreal, Quebec, Canada; Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec, Canada.
The Journal of biological chemistry
|July 3, 2023
概括
这项研究描述了Aspergillus niger中的β-ketoadipate通路,识别了原卡特丘酸分解的关键基因. 这有助于我们更好地了解真菌芳香化合物的代谢和素的价值化.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 代谢工程是代谢工程.
背景情况:
- 菌类和细菌通过二化芳物将芳香化合物代谢成TCA循环中间体.
- 对于这一过程至关重要的β-基酸盐通路在细菌中被很好地理解,但在真菌中不完全表征.
- 了解真菌通路对于素的价值化和扩大对微生物代谢的知识至关重要.
研究的目的:
- 识别和分配涉及β-基酸基因途径的基因,以在Aspergillus niger中利用Protocatechuic酸.
- 阐明芳香化合物的真菌代谢的完整代谢路径.
主要方法:
- 基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因
- 整体转录组测序以识别上调调的基因.
- 基因删除研究,以评估在protocatechuic酸上的生长.
- 使用质谱法进行代谢物分析.
- 对重组蛋白的酶测定.
主要成果:
- 五个基因 (NRRL3_01405,NRRL3_02586,NRRL3_01409,NRRL3_01886,NRRL3_01526) 被分配给原卡特丘酸降解途径中的特定酶.
- 一种基因删除突变 (ΔNRRL3_00837) 显示出对原卡特丘酸的生长受损,这表明它起着重要的作用.
- 尽管NRRL3_00837的功能至关重要,但其功能仍未确定.
结论:
- 鉴定出了在阿斯伯吉路斯尼日尔菌中编码原形酸3,4-二氧化酶,3-碳氧-cis,cis-酸盐环酶,3-碳氧-muconolactone氧化酶/脱碳氧化酶,β-ketoadipate:succinyl-CoA转移酶和β-ketoadipyl-CoA thiolase的基因.
- 这项研究提供了对真菌芳香化合物代谢的基本理解,这对于生物技术应用,如素价值化至关重要.
关键词:
3,4-二二基酸 3,4-二二基酸是一种亚斯珀吉洛斯 (Aspergillus) 是一种植物.催化剂的催化作用是什么酶动力学 酶动力学菌是一种真菌.基因淘汰赛 基因淘汰赛甲基甲基酸是什么? 甲基甲基甲酸是什么?翻译学 翻译学 翻译学 翻译学基基酸 (β-ketoadipate) 是一种基基酸.更多相关视频
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