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评估EUCAST RAST使用日本可用的抗微生物盘的可行性

Ayumi Uechi¹, Kohei Uechi¹, Ami Nakano¹

¹Division of Clinical Laboratory and Blood Transfusion, University of the Ryukyus Hospital, Okinawa, Japan.

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy

|July 3, 2023

概括

快速抗微生物敏感性测试 (RAST) 对日本的E. coli和K. pneumoniae等临床分离物有希望. 然而,为了使用可用的抗菌素盘获得准确的结果,需要对 RAST 断点进行调整.

科学领域:

临床微生物学临床微生物学
传染性疾病传染性疾病
抗微生物耐药性抗微生物耐药性

背景情况:

欧洲抗微生物敏感性测试委员会 (EUCAST) 在2018年推出了快速抗微生物敏感性测试 (RAST).
RAST可以直接在阳性血液培养样本上进行.
由于药物度不同,欧卡斯特拉斯特与日本抗微生物盘的适用性需要评估.

研究的目的:

评估EUCAST RAST的可行性,使用日本随时可用的抗菌磁盘.
将RAST结果与标准自动化抗微生物敏感性测试 (AST) 方法进行比较.

主要方法:

127个临床隔离物 (65种大肠杆菌,62种肺炎) 通过使用RAST与塞福他西姆,塞夫塔齐迪姆,美罗和西普罗夫洛克萨辛盘进行了测试.
结果与自动化AST系统 (VITEK®2) 相比较.

主要成果:

对于RAST的整体类别一致性在化时间中很高 (95.696.8%).
在大肠杆菌中观察到塞夫塔齐RAST和在肺炎中观察到塞夫塔齐RAST的显著错误,具有特定的磁盘类型和潜伏时间.

结论:

欧卡斯特拉斯特证明了在日本对大肠杆菌和K. pneumoniae敏感性测试的潜在实用性.
为了确保准确性,对一些抗微生物药物来说,需要修改 RAST 断点.

关键词:

血液培养血液培养是什么?快速抗菌感应性测试 (RAST) 是一种快速的抗菌感应性测试.欧洲抗生素敏感性测试委员会 (EUCAST)

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