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在Xenopus tropicalis模型中,青春期的性发育和受精子生成中断的终点

Sofie Svanholm¹, Mauricio Roza², Daniele Marini³

¹Department of Environmental Toxicology, Uppsala University, Uppsala 754 36, Sweden.

Reproductive toxicology (Elmsford, N.Y.)

|July 3, 2023

概括

这项研究引入了Xenopus tropicalis作为评估抗雄激素内分泌干扰物的模型. 它揭示了青精子生成和青春期开始的新见解,表明了新的毒理学终点.

科学领域:

环境毒理学环境毒理学
生殖毒理学生殖毒理学
两动物生物学

背景情况:

抗雄激素内分泌干扰化学物质 (EDC) 对野生动物构成风险.
在评估EDC效应方面,周周青春期毒理学模型至关重要.
热带 (Xenopus tropicalis) 为生殖毒理学研究提供了一个有价值的模型.

研究的目的:

建立Xenopus tropicalis作为评估抗雄激素EDCs的近青春期模型.
为了描述青少年X. tropicalis. 的性成熟.
为了研究短时间暴露于胺胺对生殖发育的影响.

主要方法:

年轻的Xenopus tropicalis被暴露在不同度的花胺 (0.250,500,1000μg/L) 中,持续2.5周.
进行了生殖腺和米勒尔管道的组织学分析.
识别新的精子阶段,包括浅色和深色精子干细胞 (SSC).

主要成果:

对照男性丸显示精子,表明青春期开始;卵巢不成熟.
500μg/L的弗拉塔胺暴露减少了深色SSC和增加了二次精子.
没有观察到对卵巢或穆勒导管的显著影响.

结论:

这项研究提供了关于Xenopus tropicalis的精子生成和青春期开始的新数据.
对于内分泌和生殖毒理学试验,提出了评估精子生成的新终点.
热带 (Xenopus tropicalis) 是评估抗雄激素EDC对男性生殖发育影响的合适模型.

关键词:

抗雄激素是一种抗雄激素.穆勒的管道是穆勒的管道.产卵生成 (Oogenesis) 是一种生殖能力的发展生殖能力的发展精子发生的过程是精子生成.它们是Xenopus tropicalis.

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