羊的增强的粘膜反应藏β-Aβ-环球蛋白对抗血红病
Cintia Hiromi Okino1, Simone Cristina Méo Niciura1, Paula Roberta Giaretta2
1Embrapa Pecuária Sudeste, São Carlos, São Paulo, Brazil.
Parasite immunology
|July 3, 2023
概括
具有βA等位基因的绵羊对血红病具有更好的抵抗力,由更高的包装细胞体积 (PCV) 表明. 这种耐药性与腹腔内增强的局部免疫反应有关,特别是Th2活性和粘素生产.
科学领域:
- 兽医免疫学 兽医免疫学
- 寄生虫学的寄生虫学
- 动物遗传学动物遗传学
背景情况:
- Ovine β-globin 多态性与通过氧 afinity 变化与血红病耐药性有关.
- 参与这种抗药机制的局部宿主反应在很大程度上仍未被研究.
研究的目的:
- 为了评估表型参数和局部腹腔响应在不同β-环球蛋白类型的羊自然感染海蒙丘斯轮.
- 为了研究β-环球蛋白单元类型与对临床血红病的抗性/弹性之间的关系.
主要方法:
- 对63,84和105天大年龄的莫拉达诺瓦羔羊 (n=2) 进行便卵数和包装细胞体积 (PCV) 的监测.
- 在210天内抽取腹腔组织样本,用于显微镜损伤评估和基因表达分析 (免疫,粘素,乳素活动).
- 在Hb-AA和Hb-BBβ-环球因子类型的羊之间进行比较.
主要成果:
- 与Hb-BB相比,带有βA等位基因 (Hb-AA) 的羔羊表现出更好的抗性/弹性,在H. contortus感染期间保持更高的PCV.
- Hb-AA动物表现出增加的腹腔叶绿素,更高的Th2配置文件,粘素和乳素活性转录.
- Hb-BB动物在腹腔中表现出更明显的炎症反应.
结论:
- β-环球蛋白单体型的βA等位基因与绵羊对临床血红病的抗性和弹性增强有关.
- 这项研究提供了第一个证据,证明了与βA等位基相关联的初级感染部位 (腹腔) 的增强局部免疫反应.
关键词:
海蒙丘斯 (Haemonchus contortus) 是一个长长的鱼类.RT-qPCRR 是一个人.这就是abomasum abomasum的意思.哈普洛型是指一个类型.乳清素是指乳清素的一种成分.一个叫做mucincin的孩子.羊类 羊类 羊类 羊类更多相关视频
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