来自培养人类口腔细菌的细菌参考基因组目录
Wenxi Li1,2, Hewei Liang1, Xiaoqian Lin1,2
1BGI-Shenzhen, 518083, Shenzhen, China.
NPJ biofilms and microbiomes
|July 3, 2023
概括
这项研究介绍了培养口腔细菌基因组参考 (COGR) 数据库,提供1089种口腔细菌的高质量基因组. 本资源有助于理解口腔微生物群的多样性及其与诸如类风湿性关节炎等健康状况的联系.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 口腔健康 口腔健康
背景情况:
- 人口腔拥有多样化的微生物群落,但只有有限数量的孤立物种和高质量的基因组可用.
- 了解口腔微生物群对于确定其在健康和疾病中的作用至关重要.
研究的目的:
- 创建一个全面的参考数据库培养人类口腔细菌基因组.
- 描述口腔微生物组的基因组内容和多样性.
- 探索口腔细菌与类风湿性关节炎等疾病之间的潜在联系.
主要方法:
- 从牙斑块,舌头和唾液中进行大规模的有氧和无氧培养人类口腔细菌.
- 全基因组测序和培养细菌的组装.
- 用于分类学注释的生物信息分析,基因含量分析 (例如,CAZymes,定数感应途径) 和特定人群的特定集群的识别.
主要成果:
- 建立了培养口腔细菌基因组参考 (COGR) 数据库,包含1089个高质量的细菌基因组.
- COGR跨越了五个族群,包括195个物种级别的群,其中95个群 (315个基因组) 代表了缺乏先前分类学注释的物种.
- 发现,口腔微生物群中的很大一部分 (111个群) 是个体特有的.
- 编码碳水化合物活性酶 (CAZymes) 的基因丰富.
- 链球菌种类普遍存在,许多具有与生物膜形成相关的质量检测途径.
- 在风湿性关节炎患者中,特定的细菌集群得到了丰富.
结论:
- COGR数据库为深入研究口腔微生物组提供了宝贵的资源.
- 基于文化的隔离对于描述新型口腔细菌及其功能至关重要.
- 口腔微生物组的组成在个体之间有很大差异,可能与类风湿性关节炎等全身性疾病有关.
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