通过Azotobacter chroococcum MTCC 3858通过花生作为林氏纤维素原料通过使用资源表面方法的多基丁酸盐生物合成
Kasilingam Nagajothi1,2, A G Murugesan3
1Sri Paramakalyani Centre of Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, 627412, Tamil Nadu, India. nagajothi2011@gmail.com.
Scientific reports
|July 3, 2023
概括
地瓜水解剂有效支持由阿佐托巴克特 (Azotobacter chroococcum) 进行的多基酸盐 (PHB) 生物合成. 预处理显著提高了糖产量,导致PHB生产增加了四倍,并为化石燃料塑料提供了可持续的替代品.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物材料科学 生物材料科学
背景情况:
- 农业废物,如地贝,是处置的挑战.
- 聚乙酸 (PHB) 是一种可生物降解的聚合物,有可能取代基于化石燃料的塑料.
- 开发具有成本效益和可持续的PHB生产方法至关重要.
研究的目的:
- 评价使用Azotobacter chroococcum进行PHB生物合成的原料是花生水解剂.
- 通过中央复合设计 (RSM-CCD) 的响应表面方法来优化PHB生产.
- 评估产生的PHB的物理化学特性.
主要方法:
- 用硫酸和酶性水解预处理了花生,以增强糖分的释放.
- Azotobacter chroococcum MTCC 3853是在浸泡发酵 (SMF) 条件下进行培养的.
- 使用RSM-CCD优化发酵参数,包括基质度,源,pH,温度和化时间.
主要成果:
- 与未经处理的样本相比,预处理显著增加了花生的糖产量.
- 在优化条件下,最大生物质的产量为17.23 g/l,PHB的产量为11.46 g/l (66.51 wt% DCW).
- 与未经处理的相比,PHB产量在预处理的花生水解剂中增加了四倍.
结论:
- 核桃水解剂是PHB生产的可行且具有成本效益的原料.
- 这种方法提供了一种有效的农业废弃物利用方法.
- 这项研究支持使用像PHB这样的生物塑料来减少对石油塑料的依赖.
相关概念视频
01:26Biosynthesis of Polysaccharides
40
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
40
01:29Biosynthesis of Lipids
40
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
40
01:24Biosynthesis in Bacteria
41
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
41