印地带 zona pellucida对卵子细胞生长和发育的影响,从累积细胞基因表达的变化中进行了探索
Jingyi Hu1, Huihui Wang1, Ran Jiang1
1Center for Reproductive Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Archives of gynecology and obstetrics
|July 3, 2023
概括
人类卵子细胞中入的透光区 (iZP) 可能会阻碍颗粒细胞和卵子细胞之间的通信. 这种受损的交换会影响卵细胞的发育,并导致不孕,这表明了治疗的新途径.
科学领域:
- 生殖生物学 生殖生物学
- 细胞和分子生物学是细胞和分子生物学.
- 基因组学就是基因组学.
背景情况:
- 人体卵细胞中异常的光透区 (ZP),特别是缩的ZP (iZP),是不孕不育的重要原因,目前没有有效的治疗方法.
- 了解iZP背后的分子机制对于开发新的治疗策略至关重要.
研究的目的:
- 为了研究印地带 zona pellucida (iZP) 对颗粒细胞 (GC) 基因表达的影响.
- 通过分析GC转录组,探索iZP如何影响卵细胞生长和发育.
主要方法:
- 在ICSI周期中,从具有iZP和正常ZP的卵细胞中采集了粒状细胞 (GC).
- 下一代RNA测序 (RNAseq) 用于GC的转录组分析.
主要成果:
- RNAseq确定了177个来自ZP和正常ZP卵细胞的GC之间的差异表达基因 (DEG).
- 参与免疫反应 (CD274),炎症 (IL4R,IL-7R),卵细胞发育途径 (Hippo,PI3K-AKT,Ras,信号) 和细胞粘附 (cadherin家族) 的关键基因在iZP病例中显著下调.
- 神经营养素信号通路组件 (NTRK2,BDNF,NT5E) 对于卵细胞支持至关重要,也被下调.
结论:
- 特定基因的下调表明ZIP中的细胞间通信和GC与卵细胞之间的物质交换受损.
- 这些干扰可能会阻碍卵细胞的生长和发育,导致与iZP相关的不孕症.
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