相关实验视频

Updated: Jul 24, 2025

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma

A Three-Dimensional Spheroid Model to Investigate the Tumor-Stromal Interaction in Hepatocellular Carcinoma

Published on: September 30, 2021

hsa_circ_0002003的升级促进了肝细胞癌的进展

Lisha Zhou^1,2,3,4, Qianwen Wang^1,2,3, Jun Hou^1,2,3

¹NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang, China.

|July 3, 2023

概括

循环RNAs (circRNAs) 是肝细胞癌 (HCC) 的关键. Hsa_circ_0002003在HCC上调,促进细胞生长和转移,并可作为预后生物标志物.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

循环RNAs (circRNAs) 越来越多地被认为是它们在人类恶性瘤中的作用.
它们代表了各种癌症的有希望的生物标志物,包括肝细胞癌 (HCC).

研究的目的:

为了研究HCC.中独特的circRNA表达特征.
确定与HCC发育和进展相关的新型circRNA生物标志物.

主要方法:

在HCC组织中的差异性circRNA表达分析.
在体外功能测试使用候选circRNAs的过度表达和siRNA向.
预测circRNA-miRNA相互作用和下游基因标,随后进行生存分析和qRT-PCR.

主要成果:

确定并验证了三个上调的环RNA (hsa_circ_0002003,hsa_circ_0002454,hsa_circ_0001394) 和一个下调的环RNA (hsa_circ_0003239).
在hsa_circ_0002003的上调促进了hcc细胞的生长和体外转移.
建立了hsa_circ_0002003/hsa-miR-1343-3p/STMN1监管轴,其中STMN1下调与HCC预后不佳相关.

结论:

Hsa_circ_0002003在HCC的发病过程中起着至关重要的作用.
Hsa_circ_0002003 是 HCC 的一个潜在的预后生物标志物.
针对hsa_circ_0002003/hsa-miR-1343-3p/STMN1轴为HCC提供了一个潜在的治疗策略.

关键词:

生物标志物生物标志物这是循环RNARNA.肝癌是一种肝癌.预测预后预测预测

更多相关视频

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment — Challenges and Innovations in Cancer Prognosis

Author Spotlight: Investigating Immune Cell Dynamics in the Tumor Microenvironment — Challenges and Innovations in Cancer Prognosis

Published on: April 12, 2024

相关概念视频

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...