在基底二氧化基中合成酶的分裂进化
Paula Sophie Seibold1,2, Stefanie Lawrinowitz1,2, Ihar Raztsou3
1Institute of Pharmacy, Department Pharmaceutical Microbiology, Friedrich Schiller University Jena, Winzerlaer Strasse 2, 07745, Jena, Germany.
Fungal biology and biotechnology
|July 3, 2023
概括
对于多酸和阿特罗门丁的巴西迪奥米塞特类合成酶,尽管有类似的机制,但它们的演变是独立的. 研究人员确定了关键的氨基酸,以设计酶基质的特异性,从而实现双重生产.
科学领域:
- 生物化学 生物化学
- 菌类学 菌类学是指菌类学.
- 自然产品 化学 化学
背景情况:
- 甲基是重要的基础基因组天然产品.
- 这些化合物通过调节细菌生物膜和机动性来影响微生物联盟.
- 这项研究调查了对关键烯基诺因负责的类合成酶的起源.
研究的目的:
- 为了确定聚酸和阿特罗门合成酶的遗传起源.
- 了解这些酶的进化和基质特异性.
- 为未来的酶工程奠定了基础.
主要方法:
- 在Aspergilli中复制Hapalopilus rutilans和Psilocybe cubensis合成酶活动.
- 液体染色学和质谱学用于酶识别.
- 生物信息学用于家族遗传重建和向氨基酸替代.
主要成果:
- 确定了HapA1,HapA2和PpaA1作为多酸合成酶.
- 证明了聚酸和阿特罗门丁合成酶的独立进化,具有共享的催化机制.
- 能够通过氨基酸修饰产生这两种化合物的工程双功能合成酶.
结论:
- 氨酸合成酶在基础基酶中独立地进化了两次,与芳香的α-基酸基质联系在一起.
- 识别和改变关键氨基酸残留物以实现放松的基质特异性.
- 建立了针对类合成酶的向酶工程的基础.
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