病毒样颗粒的细胞内传递使用可脱落的链接器
Laurel M Hagge1, Arezoo Shahrivarkevishahi1, Noora M Al-Kharji1
1Department of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, Texas 75080, USA. Gassensmith@utdallas.edu.
Journal of materials chemistry. B
|July 4, 2023
概括
研究人员开发了一种用于药物输送的新型纳米粒子载体. 这种载体有效地逃脱了内分泌体,并将货物送到细胞质中,克服了纳米疗法的一个主要挑战.
科学领域:
- 生物技术是生物技术.
- 纳米医学是一种纳米医学.
- 药物运输 药物运输 药物运输
背景情况:
- 细胞内药物输送对于治疗至关重要,但受到内体陷和 lysosomal 降解的阻碍.
- 由于细胞防御机制,纳米疗法在达到细胞质方面面临挑战.
研究的目的:
- 设计和合成一种功能性纳米载体,能够进行内体体逃脱和有效的细胞质递送.
- 克服纳米治疗药物细胞内传输的局限性.
主要方法:
- 一种对醇敏感的马列胺链体的化学合成,将三酸 (TPP) 连接到类似病毒的粒子 (VLP) Qβ.
- 利用TPP用于内体膜破坏和细胞质中细胞质中通过谷氨介导的链裂变.
- 在功能化的VLP中封装模型蛋白 (GFP,smURFP) 和siRNA (siLuc).
主要成果:
- 在肺腺癌细胞和小鼠肺上皮细胞中证明了载有GFP (体外) 和smURFP (体内) 的VLP的成功细胞溶液输送.
- 观察到由TPP部分介导的高效内体逃逸.
- 与对照组相比,使用带有siRNA的VLP和可切割的链接器展示了增强的基因沉默疗效.
结论:
- 开发了一种基于VLP的新型纳米载体,配有可抛弃的TPP链接器,用于向的细胞溶液输送.
- 设计的系统有效地克服了内体障碍,并实现了细胞质中治疗载荷的释放.
- 这种方法有望提高细胞内输送纳米治疗药物的疗效.
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