结合性内牙-牙周疾病中的微生物群及其对内心炎的风险
Brenda P F A Gomes1, Vanessa B Berber2, Vito M Chiarelli-Neto2
1Division of Endodontics, Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas-UNICAMP, Av. Limeira 901, Bairro Areao, Piracicaba, São Paulo, 13414-903, Brazil. bpgomes@fop.unicamp.br.
Clinical oral investigations
|July 4, 2023
概括
结合性内-牙周病变中的口腔细菌与传染性内心炎 (IE) 和其他全身性疾病有关. 这项研究使用下一代测序 (NGS) 来识别这些细菌并预测抗菌素耐药性.
科学领域:
- 口腔微生物学 口腔微生物学
- 感染性内心炎感染性内心炎
- 下一代测序 (NGS) 是指下一代的测序.
背景情况:
- 传染性内心炎 (IE) 具有显著的死亡风险,口腔微生物群被确定为主要风险因素.
- 结合性内膜-牙周病变 (EPL) 代表了一个独特的口腔环境,可能含有IE相关的病原体.
研究的目的:
- 评估EPL患者根管 (RC) 和牙周口袋 (PP) 样本中的微生物群.
- 使用下一代测序 (NGS) 识别与IE相关的细菌物种.
主要方法:
- 从15名患者的RC和PP收集的微生物样本,以及具有生命脉的RC (负控).
- 基因组分析结合生物信息学和IE相关细菌数据库.
- 使用PICRUSt2进行功能预测,以评估微生物社区的作用.
主要成果:
- 已经确定了主要的属:帕维莫纳斯,链球菌和菌菌.
- 来自RC的34种和来自PP的53种与IE有关.
- 与全身性疾病 (如心肌炎,亨廷顿病) 相关的微生物群概况和预测的抗菌素耐药性.
结论:
- 在EPL中的微生物不仅是IE的潜在风险因素,也是系统性疾病的潜在风险因素.
- 在PICRUSt2分析中推断出抗菌素对广谱药物的耐药性.
- NGS和生物信息学是微生物社区研究和感染诊断的强大工具.
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