变异,食品级表达,和一个乳酶的特征为zearalenone降解
Yangyu Qiu1,2, Huidong Xu2, Qinyi Ji2
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Applied microbiology and biotechnology
|July 4, 2023
概括
工程乳酶Zhd101.1表现出对泽拉伦 (ZEN) 生物降解的增强活性和稳定性. 这种食品级酶在溶液和受污染的玉米中显示出显著的ZEN降解,为消除真菌毒素提供了一个有前途的策略.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 食品安全 食品安全
背景情况:
- 泽拉伦 (ZEN) 是一种普遍存在的真菌毒素,对人类健康构成重大风险.
- 对于消除ZEN的高效和环保方法至关重要.
- 来自Clonostachys rosea的乳酶Zhd101显示了ZEN水解的潜力.
研究的目的:
- 通过向突变增强Zhd101乳酶的应用特性.
- 开发一种食品级的重组酵母系统,用于工程酶的表达和分泌.
- 评估突变酶的酶性质和ZEN降解效率.
主要方法:
- 在Zhd101基因中引入了组合突变,以创建增强的变体.
- 最佳突变物 (Zhd101.1) 用食品级酵母Kluyveromyces lactis GG799.9进行表达和分泌.
- 酶性质 (活性,稳定性) 和ZEN降解在溶液和ZEN污染的玉米样本中进行了评估.
主要成果:
- 与野生类型酶相比,工程突变Zhd101.1的特定活性增加了1.1倍.
- Zhd101.1 显示了更好的热稳定性和pH稳定性.
- 在优化条件下,含有Zhd101.1的发酵超剂在溶液中达到96.9%的ZEN降解,在玉米样本中达到74.6%.
结论:
- 工程Zhd101.1酶具有卓越的酶特性和显著的ZEN生物降解能力.
- 食品级重组酵母菌株为产生突变酶提供了一个有效的平台.
- 在食品和料行业中,Zhd101.1是开发zearalenone生物降解技术的有希望的生物催化剂.
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