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Sperm Collection of Differential Quality Using Density Gradient Centrifugation

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Published on: November 29, 2018

玻璃羊毛柱过用于马精子选择:与单层合体离心法进行比较分析

Giuliana Elizabeth Zevallos Valenzuela¹, Alejandro Ferrante^1,2, Gustavo Luis Verón¹

¹Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina (CONICET), Fundación IBYME (FIBYME), Buenos Aires, Argentina.

Reproduction in domestic animals = Zuchthygiene

|July 4, 2023

概括

玻璃羊毛柱过 (GWCF) 从新鲜和冷样本中有效地选择高质量的马精子,为标准的合体离心法提供简单,低成本的替代方案. 这种方法在精子运动性和形态学方面表现出类似或优异的结果.

科学领域:

兽医繁殖兽医繁殖兽医繁殖
精子选择技术精子选择技术
马精液分析马精液分析

背景情况:

使用Androcoll-ETM进行体离心是马类精子选择的标准.
对马精子的玻璃羊毛柱过 (GWCF) 的报告有限.
评估替代性,具有成本效益的精子选择方法对于马类繁殖至关重要.

研究的目的:

评估GWCF (50和75毫克列) 对于选择优质马精的疗效.
将GWCF的性能与Androcoll-ETM离心法进行比较,用于新鲜和冷解的精液.
评估精子的关键参数,包括运动性,形态和透性抵抗.

主要方法:

精液样本 (新鲜和冷解) 经过GWCF (50毫克和75毫克列) 和Androcoll-ETM离心.
精子质量通过确定总运动性 (TM),渐进运动性 (PM),形态正常 (MN),透能力 (HOS+) 和体完好/透能力 (AI/HOS+) 的精子的百分比来评估.
在不同方法之间还比较了精子回收数量.

主要成果:

与对照组相比,GWCF-75在新鲜精液中显著改善了PM,MN和HOS+精子.
对于冷解的精子,GWCF-75增强的TM,PM,MN,HOS+和AI/HOS+精子.
在大多数参数上,GWCF的表现与Androcoll-ETM相当或优于它,精子恢复率与Androcoll-ETM相似.

结论:

GWCF是一种可行的,简单和低成本的方法,用于选择高质量的马精.
GWCF的有效性与目前的安德罗科尔-ETM离心法标准可比.
GWCF为改善马类人工授精计划提供了一个有希望的替代方案.

关键词:

安德罗科尔-ETM合物离心法结的冷的冷的冷玻璃羊毛柱子过的过方法马的马,马的马,马的马,马的马.精子选择精子选择精子选择精子精子精子马匹马匹的马匹马匹

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