与壁相关的激酶BrWAK1赋予了Brassica rapa中对菌的抵抗力
Bin Zhang1,2,3,4, Tongbing Su1,2,3,4, Xiaoyun Xin1,2,3,4
1Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences (BAAFS), Beijing, China.
Plant biotechnology journal
|July 4, 2023
概括
一个新的壁相关激酶1 (WAK1) 基因,BrWAK1,增强了中国白菜对菌的抵抗力. 这一发现加快了对抗病作物的育种,而不会影响植物生物质.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物病理学 植物病理学
- 作物遗传学作物遗传学
背景情况:
- 植物细胞壁对防御环境压力和病原体至关重要.
- 壁相关激酶 (WAKs) 是感知细胞壁变化和调解防御反应的关键参与者.
- 黄菌 (Hyaloperonospora brassicae) 在中国白菜 (Brassica rapa L. ssp. 人 (Pekinensis) 是一种植物.
研究的目的:
- 识别和描述一个与墙壁相关的激酶 (WAK) 基因,使中国白菜对菌产生抵抗力.
- 研究BrWAK1在植物防御机制中的作用及其与其他信号通路的相互作用.
- 提供加速繁殖计划的基础,以提高中国白菜的菌耐药性.
主要方法:
- 在双倍单 haploid 种群中识别了候选耐药 WAK 基因 (BrWAK1).
- 对 BrWAK1 基因表达反应对酸和病原体注射的分析.
- 通过敏感和耐药系的基因表达的功能验证,包括截断的变异.
- 研究BrWAK1和BrBAK1.1之间的蛋白质与蛋白质相互作用.
- 对下游信号通路的分析,包括基因激活蛋白激酶 (MAPK) 级联.
主要成果:
- 一个新的WAK基因,BrWAK1,在一个主要的抗性定量特征位点中被确定.
- BrWAK1的表达是由酸和病原体的攻击引起的.
- 过度表达BrWAK1在易感线增强了对菌的抵抗力,而在耐药线中切断它增加了易感性.
- BrWAK1的细胞外银河结合 (GUB) 域对于赋予耐药性至关重要.
- BrWAK1与BrBAK1相互作用,激活了用于防御信号的MAPK级联.
- BrWAK1不会显著影响植物生物质.
结论:
- BrWAK1是第一个特征的WAK基因,在中国白菜中赋予了显著的菌耐药性.
- BrWAK1通过与BrBAK1相互作用来激活MAPK信号,增强植物防御功能.
- BrWAK1代表了一种有价值的遗传资源,可以有效地开发抗病的中国白菜品种.
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